Titill: | Noncovalent and site-directed spin labeling of duplex RNA |
Höfundur: |
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Útgáfa: | 2016 |
Tungumál: | Enska |
Umfang: | 14442-14445 |
Háskóli/Stofnun: | Háskóli Íslands University of Iceland |
Svið: | Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) |
Deild: | Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) Raunvísindastofnun (HÍ) Science Institute (UI) |
Birtist í: | Chemical Communications;52(100) |
ISSN: | 1359-7345 1364-548X (eISSN) |
DOI: | 10.1039/C6CC08387K |
Efnisorð: | Nicotinamide adenine dinucleotide; Electron paramagnetic resonance; Nucleic acid structure; EPR spectroscopy; Isoindoline nitroxides; Alcohol dehydrogenase; Distance measurements; Abasic sites; DNA; Dynamics; Litrófsgreining; Kjarnsýrur; Rafeindir |
URI: | https://hdl.handle.net/20.500.11815/1960 |
Útdráttur:An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, G is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
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Athugasemdir:Post-print (lokagerð höfundar)
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