Noncovalent and site-directed spin labeling of duplex RNA

Title:	Noncovalent and site-directed spin labeling of duplex RNA
Author:	Kamble, Nilesh Ramesh Gränz, Markus Prisner, Thomas F. Sigurdsson, Snorri orcid.org/0000-0003-2492-1456 4 authors Show fewer authors
Date:	2016
Language:	English
Scope:	14442-14445
University/Institute:	Háskóli Íslands University of Iceland
School:	Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI)
Department:	Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) Raunvísindastofnun (HÍ) Science Institute (UI)
Series:	Chemical Communications;52(100)
ISSN:	1359-7345 1364-548X (eISSN)
DOI:	10.1039/C6CC08387K
Subject:	Nicotinamide adenine dinucleotide; Electron paramagnetic resonance; Nucleic acid structure; EPR spectroscopy; Isoindoline nitroxides; Alcohol dehydrogenase; Distance measurements; Abasic sites; DNA; Dynamics; Litrófsgreining; Kjarnsýrur; Rafeindir
URI:	https://hdl.handle.net/20.500.11815/1960
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Abstract: An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, G is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
Description: Post-print (lokagerð höfundar)