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Noncovalent and site-directed spin labeling of duplex RNA

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Kamble, Nilesh Ramesh
dc.contributor.author Gränz, Markus
dc.contributor.author Prisner, Thomas F.
dc.contributor.author Sigurdsson, Snorri
dc.date.accessioned 2020-08-10T15:21:54Z
dc.date.available 2020-08-10T15:21:54Z
dc.date.issued 2016
dc.identifier.issn 1359-7345
dc.identifier.issn 1364-548X (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/1960
dc.description Post-print (lokagerð höfundar)
dc.description.abstract An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, G is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
dc.description.sponsorship This work was supported by the Deutsche Forschungsgemeinschaft DFG (SFB 902 Molecular Principles of RNA-based Regulation), the Icelandic Research Fund (141062051) and by a doctoral fellowship to N. R. K. from the University of Iceland Research Fund. We thank Dr S. Jonsdottir for assistance in collecting analytical data for structural characterization of the compounds.
dc.format.extent 14442-14445
dc.language.iso en
dc.publisher Royal Society of Chemistry (RSC)
dc.relation.ispartofseries Chemical Communications;52(100)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Nicotinamide adenine dinucleotide
dc.subject Electron paramagnetic resonance
dc.subject Nucleic acid structure
dc.subject EPR spectroscopy
dc.subject Isoindoline nitroxides
dc.subject Alcohol dehydrogenase
dc.subject Distance measurements
dc.subject Abasic sites
dc.subject DNA
dc.subject Dynamics
dc.subject Litrófsgreining
dc.subject Kjarnsýrur
dc.subject Rafeindir
dc.title Noncovalent and site-directed spin labeling of duplex RNA
dc.type info:eu-repo/semantics/article
dc.description.version Peer reviewed
dc.identifier.journal Chemical Communications
dc.identifier.doi 10.1039/C6CC08387K
dc.relation.url http://pubs.rsc.org/en/content/articlepdf/2016/CC/C6CC08387K
dc.contributor.department Raunvísindadeild (HÍ)
dc.contributor.department Faculty of Physical Sciences (UI)
dc.contributor.department Raunvísindastofnun (HÍ)
dc.contributor.department Science Institute (UI)
dc.contributor.school Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school School of Engineering and Natural Sciences (UI)

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