Noncovalent and site-directed spin labeling of duplex RNA

Kamble, Nilesh Ramesh; Gränz, Markus; Prisner, Thomas F.; Sigurdsson, Snorri

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dc.contributor	Háskóli Íslands
dc.contributor	University of Iceland
dc.contributor.author	Kamble, Nilesh Ramesh
dc.contributor.author	Gränz, Markus
dc.contributor.author	Prisner, Thomas F.
dc.contributor.author	orcid.org/0000-0003-2492-1456 Sigurdsson, Snorri
dc.date.accessioned	2020-08-10T15:21:54Z
dc.date.available	2020-08-10T15:21:54Z
dc.date.issued	2016
dc.identifier.issn	1359-7345
dc.identifier.issn	1364-548X (eISSN)
dc.identifier.uri	https://hdl.handle.net/20.500.11815/1960
dc.description	Post-print (lokagerð höfundar)
dc.description.abstract	An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, G is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
dc.description.sponsorship	This work was supported by the Deutsche Forschungsgemeinschaft DFG (SFB 902 Molecular Principles of RNA-based Regulation), the Icelandic Research Fund (141062051) and by a doctoral fellowship to N. R. K. from the University of Iceland Research Fund. We thank Dr S. Jonsdottir for assistance in collecting analytical data for structural characterization of the compounds.
dc.format.extent	14442-14445
dc.language.iso	en
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation.ispartofseries	Chemical Communications;52(100)
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Nicotinamide adenine dinucleotide
dc.subject	Electron paramagnetic resonance
dc.subject	Nucleic acid structure
dc.subject	EPR spectroscopy
dc.subject	Isoindoline nitroxides
dc.subject	Alcohol dehydrogenase
dc.subject	Distance measurements
dc.subject	Abasic sites
dc.subject	DNA
dc.subject	Dynamics
dc.subject	Litrófsgreining
dc.subject	Kjarnsýrur
dc.subject	Rafeindir
dc.title	Noncovalent and site-directed spin labeling of duplex RNA
dc.type	info:eu-repo/semantics/article
dc.description.version	Peer reviewed
dc.identifier.journal	Chemical Communications
dc.identifier.doi	10.1039/C6CC08387K
dc.relation.url	http://pubs.rsc.org/en/content/articlepdf/2016/CC/C6CC08387K
dc.contributor.department	Raunvísindadeild (HÍ)
dc.contributor.department	Faculty of Physical Sciences (UI)
dc.contributor.department	Raunvísindastofnun (HÍ)
dc.contributor.department	Science Institute (UI)
dc.contributor.school	Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school	School of Engineering and Natural Sciences (UI)