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Noncovalent and site-directed spin labeling of duplex RNA

Noncovalent and site-directed spin labeling of duplex RNA

Title: Noncovalent and site-directed spin labeling of duplex RNA
Author: Kamble, Nilesh Ramesh
Gränz, Markus
Prisner, Thomas F.
Sigurdsson, Snorri   orcid.org/0000-0003-2492-1456
Date: 2016
Language: English
Scope: 14442-14445
University/Institute: Háskóli Íslands
University of Iceland
School: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Department: Raunvísindadeild (HÍ)
Faculty of Physical Sciences (UI)
Raunvísindastofnun (HÍ)
Science Institute (UI)
Series: Chemical Communications;52(100)
ISSN: 1359-7345
1364-548X (eISSN)
DOI: 10.1039/C6CC08387K
Subject: Nicotinamide adenine dinucleotide; Electron paramagnetic resonance; Nucleic acid structure; EPR spectroscopy; Isoindoline nitroxides; Alcohol dehydrogenase; Distance measurements; Abasic sites; DNA; Dynamics; Litrófsgreining; Kjarnsýrur; Rafeindir
URI: https://hdl.handle.net/20.500.11815/1960

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An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, G is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.


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