Title: | Nitroxide-labeled pyrimidines for non-covalent spin-labeling of abasic sites in DNA and RNA duplexes |
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Date: | 2014 |
Language: | English |
Scope: | 7366-7374 |
University/Institute: | Háskóli Íslands University of Iceland |
School: | Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) |
Department: | Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) |
Series: | Org. Biomol. Chem.;12(37) |
ISSN: | 1477-0520 1477-0539 (eISSN) |
DOI: | 10.1039/C4OB01095G |
Subject: | Cleave apurinic sites; Distance measurement; Nucleic acid; EPR Spectroscopy; Click chemistry; Fluorescence; Nucleoside; Ligand; Kjarnsýrur; Litrófsgreining; Sameindaerfðafræði |
URI: | https://hdl.handle.net/20.500.11815/1952 |
Citation:S. A. Shelke; G. B. Sandholt; S. Sigurdsson, „Nitroxide-labeled pyrimidines for non-covalent spinlabeling of abasic sites in DNA and RNA duplexes,“ in Organic & Biomolecular Chemistry, vol. 12, no.37, pp. 7366-7374 10.1039/c4ob01095g
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Abstract:Non-covalent and site-directed spin labeling gives easy access to spin-labeled nucleic acids for the study of their structure and dynamics by electron paramagnetic resonance (EPR) spectroscopy. In a search for improved spin labels for non-covalent binding to abasic sites in duplex DNA and RNA, ten pyrimidine-derived spin labels were prepared in good yields and their binding was evaluated by continuous wave (CW)-EPR spectroscopy. Most of the spin labels showed lower binding affinity than the previously reported label c towards abasic sites in DNA and RNA. The most promising labels were triazole-linked spin labels and a pyrrolocytosine label. In particular, the N1-ethylamino derivative of a triazole-linked uracil spin label binds fully to both DNA and RNA containing an abasic site. This is the first example of a spin label that binds fully through non-covalent interactions with an abasic site in RNA.
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Description:Post-print (lokagerð höfundar)
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