dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Shelke, Sandip |
dc.contributor.author |
Sandholt, Gunnar Birgir |
dc.contributor.author |
Sigurdsson, Snorri |
dc.date.accessioned |
2020-08-10T13:47:32Z |
dc.date.available |
2020-08-10T13:47:32Z |
dc.date.issued |
2014 |
dc.identifier.citation |
S. A. Shelke; G. B. Sandholt; S. Sigurdsson, „Nitroxide-labeled pyrimidines for non-covalent spinlabeling of abasic sites in DNA and RNA duplexes,“ in Organic & Biomolecular Chemistry, vol. 12, no.37, pp. 7366-7374 10.1039/c4ob01095g |
dc.identifier.issn |
1477-0520 |
dc.identifier.issn |
1477-0539 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1952 |
dc.description |
Post-print (lokagerð höfundar) |
dc.description.abstract |
Non-covalent and site-directed spin labeling gives easy access to spin-labeled nucleic acids for the study of their structure and dynamics by electron paramagnetic resonance (EPR) spectroscopy. In a search for improved spin labels for non-covalent binding to abasic sites in duplex DNA and RNA, ten pyrimidine-derived spin labels were prepared in good yields and their binding was evaluated by continuous wave (CW)-EPR spectroscopy. Most of the spin labels showed lower binding affinity than the previously reported label c towards abasic sites in DNA and RNA. The most promising labels were triazole-linked spin labels and a pyrrolocytosine label. In particular, the N1-ethylamino derivative of a triazole-linked uracil spin label binds fully to both DNA and RNA containing an abasic site. This is the first example of a spin label that binds fully through non-covalent interactions with an abasic site in RNA. |
dc.description.sponsorship |
We thank the Icelandic Research Fund (110035022) and the University of Iceland Research Fund for financial support. S. A. S. acknowledges a doctoral fellowship from the University of Iceland Research Fund. We thank Dr S. Jonsdottir for assistance in collecting analytical data for structural characterization of the compounds that were prepared and members of the Sigurdsson research group for critical reading of the manuscript. |
dc.format.extent |
7366-7374 |
dc.language.iso |
en |
dc.publisher |
Royal Society of Chemistry (RSC) |
dc.relation.ispartofseries |
Org. Biomol. Chem.;12(37) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Cleave apurinic sites |
dc.subject |
Distance measurement |
dc.subject |
Nucleic acid |
dc.subject |
EPR Spectroscopy |
dc.subject |
Click chemistry |
dc.subject |
Fluorescence |
dc.subject |
Nucleoside |
dc.subject |
Ligand |
dc.subject |
Kjarnsýrur |
dc.subject |
Litrófsgreining |
dc.subject |
Sameindaerfðafræði |
dc.title |
Nitroxide-labeled pyrimidines for non-covalent spin-labeling of abasic sites in DNA and RNA duplexes |
dc.type |
info:eu-repo/semantics/article |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
Organic and Biomolecular Chemistry |
dc.identifier.doi |
10.1039/C4OB01095G |
dc.relation.url |
http://pubs.rsc.org/en/content/articlepdf/2014/OB/C4OB01095G |
dc.contributor.department |
Raunvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Physical Sciences (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |