Opin vísindi

Fletta eftir efnisorði "EPR spectroscopy"

Fletta eftir efnisorði "EPR spectroscopy"

Röðun: Raða: Niðurstöður:

  • Icon
    Benzoyl-Protected Hydroxylamines for Improved Chemical Synthesis of Oligonucleotides Containing Nitroxide Spin Labels 
    Juliusson, Haraldur Y.; Segler, Anna-Lena J.; Sigurdsson, Snorri (Wiley, 2019-06-17)
    Oligonucleotides containing nitroxide spin labels, used in biophysical studies of nucleic acids, are frequently prepared by chemical synthesis. However, during the synthesis of spin‐labeled oligonucleotides, the nitroxides are partially reduced to the ...
  • Icon
    Dynamics of Nucleic Acids at Room Temperature Revealed by Pulsed EPR Spectroscopy 
    Gränz, Markus; Erlenbach, Nicole; Spindler, Philipp; Gophane, Dnyaneshwar B.; Stelzl, Lukas S.; Sigurdsson, Snorri; Prisner, Thomas F. (Wiley, 2018-08-13)
    The investigation of the structure and conformational dynamics of biomolecules under physiological conditions is challenging for structural biology. Although pulsed electron paramagnetic resonance (like PELDOR) techniques provide long‐range distance ...
  • Icon
    Nitroxide‐Derived N‐Oxide Phenazines for Noncovalent Spin‐Labeling of DNA 
    Juliusson, Haraldur Y.; Sigurdsson, Snorri (Wiley, 2020-07)
    Two o‐benzoquinone derivatives of isoindoline were synthesized for use as building blocks to incorporate isoindoline nitroxides into different compounds and materials. These o‐quinones were condensed with a number of o‐phenylenediamines to form ...
  • Icon
    Noncovalent and site-directed spin labeling of duplex RNA 
    Kamble, Nilesh Ramesh; Gränz, Markus; Prisner, Thomas F.; Sigurdsson, Snorri (Royal Society of Chemistry (RSC), 2016)
    An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, ...
  • Icon
    Noncovalent spin-labeling of RNA: the aptamer approach 
    Saha, Subham; Hetzke, Thilo; Prisner, Thomas F.; Sigurdsson, Snorri (Royal Society of Chemistry (RSC), 2018)
    In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) ...
  • Icon
    On the Limited Stability of BDPA Radicals 
    Mandal, Sucharita; Sigurdsson, Snorri (Wiley, 2020-05-12)
    1,3‐Bis(diphenylene)‐2‐phenylallyl (BDPA)‐based radicals are of interest as polarizing agents for dynamic nuclear polarization (DNP). For this purpose, a BDPA‐nitroxide biradical, employing a phosphodiester linkage, was synthesized. Contrary to what ...
  • Icon
    Reduction Resistant and Rigid Nitroxide Spin-Labels for DNA and RNA 
    Juliusson, Haraldur Y.; Sigurdsson, Snorri (American Chemical Society (ACS), 2020-02-27)
    Electron paramagnetic resonance (EPR) spectroscopy, coupled with site-directed spin labeling (SDSL), is a useful method for studying conformational changes of biomolecules in cells. To employ in-cell EPR using nitroxide-based spin labels, the structure ...