Titill: | Nitroxide‐Derived N‐Oxide Phenazines for Noncovalent Spin‐Labeling of DNA |
Höfundur: |
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Útgáfa: | 2020-07 |
Tungumál: | Enska |
Háskóli/Stofnun: | Háskóli Íslands University of Iceland |
Svið: | Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) |
Deild: | Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) Raunvísindastofnun (HÍ) Science Institute (UI) |
ISSN: | 1439-4227 1439-7633 (eISSN) |
DOI: | 10.1002/cbic.202000128 |
Efnisorð: | Aminoxyl radicals; EPR spectroscopy; Noncovalent spin labels; Oligonucleotides; Phenazines; Litrófsgreining; Sameindaerfðafræði |
URI: | https://hdl.handle.net/20.500.11815/1975 |
Tilvitnun:Juliusson, H. Y., & Sigurdsson, S. T. (2020). Nitroxide-derived N-oxide phenazines for noncovalent spin-labeling of DNA. ChemBioChem, doi: 10.1002/cbic.202000128
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Útdráttur:Two o‐benzoquinone derivatives of isoindoline were synthesized for use as building blocks to incorporate isoindoline nitroxides into different compounds and materials. These o‐quinones were condensed with a number of o‐phenylenediamines to form isoindoline‐phenazines in high yields. Subsequent oxidation gave phenazine‐di‐N‐oxide isoindoline nitroxides that were evaluated for noncovalent and site‐directed spin‐labeling of duplex DNA and RNA that contained abasic sites. Although only minor binding was observed for RNA, the unsubstituted phenazine‐N,N‐dioxide tetramethyl isoindoline nitroxide showed high binding affinity and selectivity towards abasic sites in duplex DNA that contained cytosine as the orphan base.
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Athugasemdir:Post-print (lokagerð höfundar)
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Leyfi:This is the peer reviewed version of the following article: [Juliusson, H. Y., & Sigurdsson, S. T. (2020). Nitroxide-derived N-oxide phenazines for noncovalent spin-labeling of DNA. ChemBioChem, doi: 10.1002/cbic.202000128], which has been published in final form at https://doi.org/10.1002/cbic.202000128. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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