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Dynamics of Nucleic Acids at Room Temperature Revealed by Pulsed EPR Spectroscopy

Dynamics of Nucleic Acids at Room Temperature Revealed by Pulsed EPR Spectroscopy


Title: Dynamics of Nucleic Acids at Room Temperature Revealed by Pulsed EPR Spectroscopy
Author: Gränz, Markus
Erlenbach, Nicole
Spindler, Philipp
Gophane, Dnyaneshwar B.
Stelzl, Lukas S.
Sigurdsson, Snorri   orcid.org/0000-0003-2492-1456
Prisner, Thomas F.
Date: 2018-08-13
Language: English
Scope: 10540-10543
University/Institute: Háskóli Íslands
University of Iceland
School: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Department: Raunvísindadeild (HÍ)
Faculty of Physical Sciences (UI)
Raunvísindastofnun (HÍ)
Science Institute (UI)
Series: Angewandte Chemie International Edition;57(33)
ISSN: 1433-7851
1521-3773 (eISSN)
DOI: 10.1002/anie.201803682
Subject: Conformational dynamics; DEER spectroscopy; DNA; EPR spectroscopy; PELDOR spectroscopy; Litrófsgreining; DNA-rannsóknir; Kjarnsýrur
URI: https://hdl.handle.net/20.500.11815/1971

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Citation:

Gränz, M., Erlenbach, N., Spindler, P., Gophane, D. B., Stelzl, L. S., Sigurdsson, S. T., & Prisner, T. F. (2018). Dynamics of Nucleic Acids at Room Temperature Revealed by Pulsed EPR Spectroscopy. 57(33), 10540-10543. doi:10.1002/anie.201803682

Abstract:

The investigation of the structure and conformational dynamics of biomolecules under physiological conditions is challenging for structural biology. Although pulsed electron paramagnetic resonance (like PELDOR) techniques provide long‐range distance and orientation information with high accuracy, such studies are usually performed at cryogenic temperatures. At room temperature (RT) PELDOR studies are seemingly impossible due to short electronic relaxation times and loss of dipolar interactions through rotational averaging. We incorporated the rigid nitroxide spin label Ç into a DNA duplex and immobilized the sample on a solid support to overcome this limitation. This enabled orientation‐selective PELDOR measurements at RT. A comparison with data recorded at 50 K revealed averaging of internal dynamics, which occur on the ns time range at RT. Thus, our approach adds a new method to study structural and dynamical processes at physiological temperature in the <10 μs time range with atomistic resolution.

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Post-print (lokagerð höfundar)

Rights:

This is the peer reviewed version of the following article: Angewandte Chemie International Edition, which has been published in final form at https://doi.org/10.1002/anie.201803682. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

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