Noncovalent spin-labeling of RNA: the aptamer approach

Title:	Noncovalent spin-labeling of RNA: the aptamer approach
Author:	Saha, Subham Hetzke, Thilo Prisner, Thomas F. Sigurdsson, Snorri orcid.org/0000-0003-2492-1456 4 authors Show fewer authors
Date:	2018
Language:	English
Scope:	11749-11752
University/Institute:	Háskóli Íslands University of Iceland
School:	Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI)
Department:	Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) Raunvísindastofnun (HÍ) Science Institute (UI)
Series:	Chemical Communications;54(83)
ISSN:	1359-7345 1364-548X (eISSN)
DOI:	10.1039/C8CC05597A
Subject:	Malachite green; EPR spectroscopy; Isoindoline nitroxides; Distance measurements; Dynamics; Ligands; DNA; Fluorescence; Molecules; Litrófsgreining; Kjarnsýrur; Flúrljómun; DNA-rannsóknir
URI:	https://hdl.handle.net/20.500.11815/1972
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Citation: Saha, S., Hetzke, T., Prisner, T. F., & Sigurdsson, S. T. (2018). Noncovalent spin-labeling of RNA: The aptamer approach. Chemical Communications, 54(83), 11749-11752. doi:10.1039/c8cc05597a
Abstract: In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron–electron double resonance (PELDOR) and fluorescence spectroscopies.
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