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| Titill: | Noncovalent spin-labeling of RNA: the aptamer approach |
| Höfundur: |
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| Útgáfa: | 2018 |
| Tungumál: | Enska |
| Umfang: | 11749-11752 |
| Háskóli/Stofnun: | Háskóli Íslands University of Iceland |
| Svið: | Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) |
| Deild: | Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) Raunvísindastofnun (HÍ) Science Institute (UI) |
| Birtist í: | Chemical Communications;54(83) |
| ISSN: | 1359-7345 1364-548X (eISSN) |
| DOI: | 10.1039/C8CC05597A |
| Efnisorð: | Malachite green; EPR spectroscopy; Isoindoline nitroxides; Distance measurements; Dynamics; Ligands; DNA; Fluorescence; Molecules; Litrófsgreining; Kjarnsýrur; Flúrljómun; DNA-rannsóknir |
| URI: | https://hdl.handle.net/20.500.11815/1972 |
Tilvitnun:Saha, S., Hetzke, T., Prisner, T. F., & Sigurdsson, S. T. (2018). Noncovalent spin-labeling of RNA: The aptamer approach. Chemical Communications, 54(83), 11749-11752. doi:10.1039/c8cc05597a
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Útdráttur:In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron–electron double resonance (PELDOR) and fluorescence spectroscopies.
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Athugasemdir:Post-print (lokagerð höfundar)
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