Noncovalent spin-labeling of RNA: the aptamer approach

Almenn færsla
dc.contributorHáskóli Íslandsen_US
dc.contributorUniversity of Icelanden_US
dc.contributor.authorSaha, Subham
dc.contributor.authorHetzke, Thilo
dc.contributor.authorPrisner, Thomas F.
dc.contributor.authorSigurdsson, Snorri
dc.contributor.departmentRaunvísindadeild (HÍ)en_US
dc.contributor.departmentFaculty of Physical Sciences (UI)en_US
dc.contributor.departmentRaunvísindastofnun (HÍ)en_US
dc.contributor.departmentScience Institute (UI)en_US
dc.contributor.schoolVerkfræði- og náttúruvísindasvið (HÍ)en_US
dc.contributor.schoolSchool of Engineering and Natural Sciences (UI)en_US
dc.date.accessioned2020-08-11T15:21:55Z
dc.date.available2020-08-11T15:21:55Z
dc.date.issued2018
dc.descriptionPost-print (lokagerð höfundar)en_US
dc.description.abstractIn the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron–electron double resonance (PELDOR) and fluorescence spectroscopies.en_US
dc.description.sponsorshipThe authors acknowledge financial support by the IcelandicResearch Fund (141062051). S. S. gratefully acknowledgesa doctoral fellowship provided by the University of Iceland.T. H. and T. F. P. would like to acknowledge the CollaborativeResearch Center 902 ‘Molecular Principles of RNA-basedRegulation’ of the German Research Foundation for funding.The authors thank Dr S. Jonsdottir for assistance with collectinganalytical data for structural characterization of the compoundsprepared, K. R. Oskarsson for his assistance with collectingfluorescence data and Dr T. Halbritter for his assistance ingenerating the molecular models. The authors thank membersof the Sigurdsson research group for critical reading of themanuscript and for helpful discussionsen_US
dc.description.versionPeer revieweden_US
dc.format.extent11749-11752en_US
dc.identifier.citationSaha, S., Hetzke, T., Prisner, T. F., & Sigurdsson, S. T. (2018). Noncovalent spin-labeling of RNA: The aptamer approach. Chemical Communications, 54(83), 11749-11752. doi:10.1039/c8cc05597aen_US
dc.identifier.doi10.1039/C8CC05597A
dc.identifier.issn1359-7345
dc.identifier.issn1364-548X (eISSN)
dc.identifier.journalChemical Communicationsen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11815/1972
dc.language.isoenen_US
dc.publisherRoyal Society of Chemistry (RSC)en_US
dc.relation.ispartofseriesChemical Communications;54(83)
dc.relation.urlhttp://pubs.rsc.org/en/content/articlepdf/2018/CC/C8CC05597Aen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMalachite greenen_US
dc.subjectEPR spectroscopyen_US
dc.subjectIsoindoline nitroxidesen_US
dc.subjectDistance measurementsen_US
dc.subjectDynamicsen_US
dc.subjectLigandsen_US
dc.subjectDNAen_US
dc.subjectFluorescenceen_US
dc.subjectMoleculesen_US
dc.subjectLitrófsgreiningen_US
dc.subjectKjarnsýruren_US
dc.subjectFlúrljómunen_US
dc.subjectDNA-rannsókniren_US
dc.titleNoncovalent spin-labeling of RNA: the aptamer approachen_US
dc.typeinfo:eu-repo/semantics/articleen_US

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