Opin vísindi

Metabolic plasticity for subcutaneous fat accumulation in a long-distance migratory bird traced by 2 H 2 O

Show simple item record

dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Viegas, Ivan
dc.contributor.author Araújo, Pedro M.
dc.contributor.author Rocha, Afonso D.
dc.contributor.author Villegas, Auxiliadora
dc.contributor.author Jones, John G.
dc.contributor.author Ramos, Jaime A.
dc.contributor.author Masero, José A.
dc.contributor.author Alves, Jose
dc.date.accessioned 2018-03-09T13:50:40Z
dc.date.available 2018-03-09T13:50:40Z
dc.date.issued 2017-01-12
dc.identifier.citation Viegas, I., Araújo, P. M., Rocha, A. D., Villegas, A., Jones, J. G., Ramos, J. A., . . . Alves, J. A. (2017). Metabolic plasticity for subcutaneous fat accumulation in a long-distance migratory bird traced by 2H2O. The Journal of Experimental Biology, 220(6), 1072-1078. doi:10.1242/jeb.150490
dc.identifier.issn 0022-0949
dc.identifier.issn 1477-9145 (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/620
dc.description.abstract The migrant black-tailed godwit (Limosa limosa) traditionally used natural wetlands in the Iberian Peninsula to prepare for migratory flights by feeding mainly in estuaries. In recent decades, this species has become increasingly dependent on rice fields, thereby relying on a plant-based diet for fuelling. Dietary fatty acids (FA) seem to be determinant to the composition of accumulated subcutaneous fat in migratory birds. It is still unclear whether metabolic plasticity allows for modification and/or synthesis of FA, contributing to a lipid profile that enables a successful migratory performance. Deuterated water was administered to captive black-tailed godwits submitted to two diets (fly larvae versus rice) and the incorporation of deuterium (2H) into subcutaneous triglycerides was analyzed by NMR. A recently developed localized biopsy method for sampling subcutaneous fat was employed with later successful release of all birds into the wild. The average chemical structure reflected mostly a mixture of saturated and monounsaturated 16- and 18-carbon FA, a profile frequently found in migrant birds. Significantly higher levels of polyunsaturated FA, as well as detectable levels of n-3 FA, were observed in fly-larvae-fed birds. Excess 2H-enrichments in FA revealed significantly higher rates of fractional de novo lipogenesis and FA desaturation capacity in rice-fed birds. This novel and non-lethal tracer method revealed the capacity of this species to alter its lipid metabolism to compensate for a poorer dietary lipid contribution. Because of its versatility, adapting this method to other scenarios and/or other migratory species is considered feasible and cost-effective.
dc.description.sponsorship The authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia (FCT) in the form of fellowships to I.V. (SFRH/BPD/90032/2012), P.M.A. (SFRH/BD/69238/2010), A.D.R. (SFRH/BD/74228/2010) and J.A.A. (SFRH/BPD/91527/2012) and research project EXCL/DTP-PIC/0069/2012. NMR data were collected at the UC-NMR facility that is supported by FEDER and FCT (RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012) and Rede Nacional de Ressonancia Magnetica Nuclear (RNRMN).
dc.format.extent 1072-1078
dc.language.iso en
dc.publisher The Company of Biologists
dc.relation.ispartofseries The Journal of Experimental Biology;220(6)
dc.rights info:eu-repo/semantics/openAccess
dc.subject NMR
dc.subject Deuterated water
dc.subject Lipogenesis
dc.subject Migration
dc.subject Lipid accumulation
dc.subject Godwits
dc.subject Jaðrakan
dc.subject Fuglafar
dc.title Metabolic plasticity for subcutaneous fat accumulation in a long-distance migratory bird traced by 2 H 2 O
dc.type info:eu-repo/semantics/article
dc.description.version Peer Reviewed
dc.identifier.journal The Journal of Experimental Biology
dc.identifier.doi 10.1242/jeb.150490
dc.contributor.department Rannsóknasetur Suðurlandi (HÍ)
dc.contributor.department Research Centre in South Iceland (UI)

Files in this item

This item appears in the following Collection(s)

Show simple item record