dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Guinde, Julien |
dc.contributor.author |
Benoit, Audrey |
dc.contributor.author |
Frankel, Diane |
dc.contributor.author |
Robert, Stéphane |
dc.contributor.author |
Ostacolo, Kevin |
dc.contributor.author |
Lévy, Nicolas |
dc.contributor.author |
Astoul, Philippe |
dc.contributor.author |
Roll, Patrice |
dc.contributor.author |
Kaspi, Elise |
dc.date.accessioned |
2021-01-28T12:27:36Z |
dc.date.available |
2021-01-28T12:27:36Z |
dc.date.issued |
2020-02-26 |
dc.identifier.citation |
Guinde J, Benoit A, Frankel D, Robert S, Ostacolo K, Lévy N, Astoul P, Roll P, Kaspi E. miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma. International Journal of Molecular Sciences. 2020; 21(5):1599. https://doi.org/10.3390/ijms21051599 |
dc.identifier.issn |
1422-0067 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/2429 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
In lung adenocarcinoma, low lamin A expression in pleural metastatic cells has been proposed as a pejorative factor. miR-9 physiologically inhibits the expression of lamin A in neural cells and seems to be a central actor in the carcinogenesis and the metastatic process in lung cancer. Thus, it could be a good candidate to explain the reduction of lamin A expression in lung adenocarcinoma cells. miR-9 expression was analyzed in 16 pleural effusions containing metastatic cells from lung adenocarcinoma and was significantly reduced in patients from the ‘Low lamin A expression’ group compared to patients from the ‘High lamin A expression’ group. Then, carcinoma cells selection by fluorescence-activated cell sorting (FACS) was performed according to epithelial membrane antigen (EMA) expression, reflecting lamin A expression. miR-9 was underexpressed in lamin A– carcinoma cells compared to lamin A+ carcinoma cells in patients from the ‘Low lamin A expression’ group, whereas there was no difference of miR-9 expression between lamin A+ and lamin A– carcinoma cells in patients from the ‘High lamin A expression’ group. These results suggest that miR-9 does not regulate lamin A expression in metastatic cells from lung adenocarcinoma. On the contrary, miR-9 expression was shown to be reduced in lamin A-negative carcinoma cells. |
dc.description.sponsorship |
This research was funded by an ARARD (Association Régionale d’Aide Respiratoire à Domicile) grant, Parc d’activités de Napollon, 100 avenue des Templiers, Aubagne Cedex, France. |
dc.format.extent |
1599 |
dc.language.iso |
en |
dc.publisher |
MDPI AG |
dc.relation.ispartofseries |
International Journal of Molecular Sciences;21(5) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Lamin A |
dc.subject |
Lung adenocarcinoma |
dc.subject |
MicroRNA-9 |
dc.subject |
Pleural effusions |
dc.subject |
Lungnasjúkdómar |
dc.title |
miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
International Journal of Molecular Sciences |
dc.identifier.doi |
10.3390/ijms21051599 |
dc.relation.url |
https://www.mdpi.com/1422-0067/21/5/1599/pdf |
dc.contributor.department |
Læknadeild (HÍ) |
dc.contributor.department |
Faculty of Medicine (UI) |
dc.contributor.department |
Lífvísindasetur (HÍ) |
dc.contributor.department |
Biomedical Center (UI) |
dc.contributor.school |
Heilbrigðisvísindasvið (HÍ) |
dc.contributor.school |
School of Health Sciences (UI) |