Titill: | miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma |
Höfundur: |
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Útgáfa: | 2020-02-26 |
Tungumál: | Enska |
Umfang: | 1599 |
Háskóli/Stofnun: | Háskóli Íslands University of Iceland |
Svið: | Heilbrigðisvísindasvið (HÍ) School of Health Sciences (UI) |
Deild: | Læknadeild (HÍ) Faculty of Medicine (UI) Lífvísindasetur (HÍ) Biomedical Center (UI) |
Birtist í: | International Journal of Molecular Sciences;21(5) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms21051599 |
Efnisorð: | Lamin A; Lung adenocarcinoma; MicroRNA-9; Pleural effusions; Lungnasjúkdómar |
URI: | https://hdl.handle.net/20.500.11815/2429 |
Tilvitnun:Guinde J, Benoit A, Frankel D, Robert S, Ostacolo K, Lévy N, Astoul P, Roll P, Kaspi E. miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma. International Journal of Molecular Sciences. 2020; 21(5):1599. https://doi.org/10.3390/ijms21051599
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Útdráttur:In lung adenocarcinoma, low lamin A expression in pleural metastatic cells has been proposed as a pejorative factor. miR-9 physiologically inhibits the expression of lamin A in neural cells and seems to be a central actor in the carcinogenesis and the metastatic process in lung cancer. Thus, it could be a good candidate to explain the reduction of lamin A expression in lung adenocarcinoma cells. miR-9 expression was analyzed in 16 pleural effusions containing metastatic cells from lung adenocarcinoma and was significantly reduced in patients from the ‘Low lamin A expression’ group compared to patients from the ‘High lamin A expression’ group. Then, carcinoma cells selection by fluorescence-activated cell sorting (FACS) was performed according to epithelial membrane antigen (EMA) expression, reflecting lamin A expression. miR-9 was underexpressed in lamin A– carcinoma cells compared to lamin A+ carcinoma cells in patients from the ‘Low lamin A expression’ group, whereas there was no difference of miR-9 expression between lamin A+ and lamin A– carcinoma cells in patients from the ‘High lamin A expression’ group. These results suggest that miR-9 does not regulate lamin A expression in metastatic cells from lung adenocarcinoma. On the contrary, miR-9 expression was shown to be reduced in lamin A-negative carcinoma cells.
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Athugasemdir:Publisher's version (útgefin grein)
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Leyfi:This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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