Noncovalent spin-labeling of RNA: the aptamer approach

Saha, Subham; Hetzke, Thilo; Prisner, Thomas F.; Sigurdsson, Snorri

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dc.contributor	Háskóli Íslands
dc.contributor	University of Iceland
dc.contributor.author	Saha, Subham
dc.contributor.author	Hetzke, Thilo
dc.contributor.author	Prisner, Thomas F.
dc.contributor.author	orcid.org/0000-0003-2492-1456 Sigurdsson, Snorri
dc.date.accessioned	2020-08-11T15:21:55Z
dc.date.available	2020-08-11T15:21:55Z
dc.date.issued	2018
dc.identifier.citation	Saha, S., Hetzke, T., Prisner, T. F., & Sigurdsson, S. T. (2018). Noncovalent spin-labeling of RNA: The aptamer approach. Chemical Communications, 54(83), 11749-11752. doi:10.1039/c8cc05597a
dc.identifier.issn	1359-7345
dc.identifier.issn	1364-548X (eISSN)
dc.identifier.uri	https://hdl.handle.net/20.500.11815/1972
dc.description	Post-print (lokagerð höfundar)
dc.description.abstract	In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron–electron double resonance (PELDOR) and fluorescence spectroscopies.
dc.description.sponsorship	The authors acknowledge financial support by the IcelandicResearch Fund (141062051). S. S. gratefully acknowledgesa doctoral fellowship provided by the University of Iceland.T. H. and T. F. P. would like to acknowledge the CollaborativeResearch Center 902 ‘Molecular Principles of RNA-basedRegulation’ of the German Research Foundation for funding.The authors thank Dr S. Jonsdottir for assistance with collectinganalytical data for structural characterization of the compoundsprepared, K. R. Oskarsson for his assistance with collectingfluorescence data and Dr T. Halbritter for his assistance ingenerating the molecular models. The authors thank membersof the Sigurdsson research group for critical reading of themanuscript and for helpful discussions
dc.format.extent	11749-11752
dc.language.iso	en
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation.ispartofseries	Chemical Communications;54(83)
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Malachite green
dc.subject	EPR spectroscopy
dc.subject	Isoindoline nitroxides
dc.subject	Distance measurements
dc.subject	Dynamics
dc.subject	Ligands
dc.subject	DNA
dc.subject	Fluorescence
dc.subject	Molecules
dc.subject	Litrófsgreining
dc.subject	Kjarnsýrur
dc.subject	Flúrljómun
dc.subject	DNA-rannsóknir
dc.title	Noncovalent spin-labeling of RNA: the aptamer approach
dc.type	info:eu-repo/semantics/article
dc.description.version	Peer reviewed
dc.identifier.journal	Chemical Communications
dc.identifier.doi	10.1039/C8CC05597A
dc.relation.url	http://pubs.rsc.org/en/content/articlepdf/2018/CC/C8CC05597A
dc.contributor.department	Raunvísindadeild (HÍ)
dc.contributor.department	Faculty of Physical Sciences (UI)
dc.contributor.department	Raunvísindastofnun (HÍ)
dc.contributor.department	Science Institute (UI)
dc.contributor.school	Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school	School of Engineering and Natural Sciences (UI)