dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Saha, Subham |
dc.contributor.author |
Hetzke, Thilo |
dc.contributor.author |
Prisner, Thomas F. |
dc.contributor.author |
Sigurdsson, Snorri |
dc.date.accessioned |
2020-08-11T15:21:55Z |
dc.date.available |
2020-08-11T15:21:55Z |
dc.date.issued |
2018 |
dc.identifier.citation |
Saha, S., Hetzke, T., Prisner, T. F., & Sigurdsson, S. T. (2018). Noncovalent spin-labeling of RNA: The aptamer approach. Chemical Communications, 54(83), 11749-11752. doi:10.1039/c8cc05597a |
dc.identifier.issn |
1359-7345 |
dc.identifier.issn |
1364-548X (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1972 |
dc.description |
Post-print (lokagerð höfundar) |
dc.description.abstract |
In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron–electron double resonance (PELDOR) and fluorescence spectroscopies. |
dc.description.sponsorship |
The authors acknowledge financial support by the IcelandicResearch Fund (141062051). S. S. gratefully acknowledgesa doctoral fellowship provided by the University of Iceland.T. H. and T. F. P. would like to acknowledge the CollaborativeResearch Center 902 ‘Molecular Principles of RNA-basedRegulation’ of the German Research Foundation for funding.The authors thank Dr S. Jonsdottir for assistance with collectinganalytical data for structural characterization of the compoundsprepared, K. R. Oskarsson for his assistance with collectingfluorescence data and Dr T. Halbritter for his assistance ingenerating the molecular models. The authors thank membersof the Sigurdsson research group for critical reading of themanuscript and for helpful discussions |
dc.format.extent |
11749-11752 |
dc.language.iso |
en |
dc.publisher |
Royal Society of Chemistry (RSC) |
dc.relation.ispartofseries |
Chemical Communications;54(83) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Malachite green |
dc.subject |
EPR spectroscopy |
dc.subject |
Isoindoline nitroxides |
dc.subject |
Distance measurements |
dc.subject |
Dynamics |
dc.subject |
Ligands |
dc.subject |
DNA |
dc.subject |
Fluorescence |
dc.subject |
Molecules |
dc.subject |
Litrófsgreining |
dc.subject |
Kjarnsýrur |
dc.subject |
Flúrljómun |
dc.subject |
DNA-rannsóknir |
dc.title |
Noncovalent spin-labeling of RNA: the aptamer approach |
dc.type |
info:eu-repo/semantics/article |
dc.description.version |
Peer reviewed |
dc.identifier.journal |
Chemical Communications |
dc.identifier.doi |
10.1039/C8CC05597A |
dc.relation.url |
http://pubs.rsc.org/en/content/articlepdf/2018/CC/C8CC05597A |
dc.contributor.department |
Raunvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Physical Sciences (UI) |
dc.contributor.department |
Raunvísindastofnun (HÍ) |
dc.contributor.department |
Science Institute (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |