Opin vísindi

A semi-rigid isoindoline-derived nitroxide spin label for RNA

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Gophane, Dnyaneshwar B.
dc.contributor.author Endeward, Burkhard
dc.contributor.author Prisner, Thomas F.
dc.contributor.author Sigurdsson, Snorri
dc.date.accessioned 2020-08-10T15:31:12Z
dc.date.available 2020-08-10T15:31:12Z
dc.date.issued 2018
dc.identifier.citation Gophane, D. B., Endeward, B., Prisner, T. F., & Sigurdsson, S. T. (2018). A semi-rigid isoindoline-derived nitroxide spin label for RNA. Organic and Biomolecular Chemistry, 16(5), 816-824 doi: 10.1039/c7ob02870a
dc.identifier.issn 1477-0520
dc.identifier.issn 1477-0539 (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/1962
dc.description Post-print (lokagerð höfundar)
dc.description.abstract A new isoindoline-derived benzimidazole nitroxide spin label, ImUm, was synthesized and incorporated into RNA oligoribonucleotides. ImUm is the first example of a conformationally unambiguous spin label for RNA, in which the nitroxide N–O bond lies on the same axis as the single bond used to attach the rigid isoindoline-based spin label to a uridine base. This results in minimal displacement of the nitroxide upon rotation of this single bond, which is a useful property for a label to be used for distance measurements. Continuous-wave (CW) EPR measurements of RNA duplexes containing ImUm indicate a restricted rotation around this single bond, presumably due to an intramolecular hydrogen bond between the benzimidazole N–H and O4 of the uracil. Orientation-selective pulsed electron–electron double resonance (PELDOR, also called double electron–electron resonance, or DEER) distance measurements between two spin labels in two RNA duplexes showed in one case a strong orientation dependence, further confirming the restricted motion of the spin labels in RNA duplexes.
dc.description.sponsorship S. Th. S. acknowledges financial support from the Icelandic Research Fund (141062-051). T. F. P. acknowledges financial support by the SFB 902 - Molecular Principles of RNA-based Regulation. We thank Dr S. Jonsdottir for assistance in collecting analytical data for structural characterization of the new compounds and Dr Subham Saha for assistance with preparation of this manuscript.
dc.format.extent 816-824
dc.language.iso en
dc.publisher Royal Society of Chemistry (RSC)
dc.relation.ispartofseries Organic & Biomolecular Chemistry;16(5)
dc.rights info:eu-repo/semantics/openAccess
dc.subject DNA-rannsóknir
dc.subject Kjarnsýrur
dc.subject Rafeindir
dc.title A semi-rigid isoindoline-derived nitroxide spin label for RNA
dc.type info:eu-repo/semantics/article
dc.description.version Peer reviewed
dc.identifier.journal Organic & Biomolecular Chemistry
dc.identifier.doi 10.1039/C7OB02870A
dc.relation.url http://pubs.rsc.org/en/content/articlepdf/2018/OB/C7OB02870A
dc.contributor.department Raunvísindadeild (HÍ)
dc.contributor.department Faculty of Physical Sciences (UI)
dc.contributor.department Raunvísindastofnun (HÍ)
dc.contributor.department Science Institute (UI)
dc.contributor.school Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school School of Engineering and Natural Sciences (UI)

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