Titill: | A semi-rigid isoindoline-derived nitroxide spin label for RNA |
Höfundur: |
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Útgáfa: | 2018 |
Tungumál: | Enska |
Umfang: | 816-824 |
Háskóli/Stofnun: | Háskóli Íslands University of Iceland |
Svið: | Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) |
Deild: | Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) Raunvísindastofnun (HÍ) Science Institute (UI) |
Birtist í: | Organic & Biomolecular Chemistry;16(5) |
ISSN: | 1477-0520 1477-0539 (eISSN) |
DOI: | 10.1039/C7OB02870A |
Efnisorð: | DNA-rannsóknir; Kjarnsýrur; Rafeindir |
URI: | https://hdl.handle.net/20.500.11815/1962 |
Tilvitnun:Gophane, D. B., Endeward, B., Prisner, T. F., & Sigurdsson, S. T. (2018). A semi-rigid isoindoline-derived nitroxide spin label for RNA. Organic and Biomolecular Chemistry, 16(5), 816-824 doi: 10.1039/c7ob02870a
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Útdráttur:A new isoindoline-derived benzimidazole nitroxide spin label, ImUm, was synthesized and incorporated into RNA oligoribonucleotides. ImUm is the first example of a conformationally unambiguous spin label for RNA, in which the nitroxide N–O bond lies on the same axis as the single bond used to attach the rigid isoindoline-based spin label to a uridine base. This results in minimal displacement of the nitroxide upon rotation of this single bond, which is a useful property for a label to be used for distance measurements. Continuous-wave (CW) EPR measurements of RNA duplexes containing ImUm indicate a restricted rotation around this single bond, presumably due to an intramolecular hydrogen bond between the benzimidazole N–H and O4 of the uracil. Orientation-selective pulsed electron–electron double resonance (PELDOR, also called double electron–electron resonance, or DEER) distance measurements between two spin labels in two RNA duplexes showed in one case a strong orientation dependence, further confirming the restricted motion of the spin labels in RNA duplexes.
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Athugasemdir:Post-print (lokagerð höfundar)
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