Opin vísindi

A semi-rigid isoindoline-derived nitroxide spin label for RNA

A semi-rigid isoindoline-derived nitroxide spin label for RNA

Title: A semi-rigid isoindoline-derived nitroxide spin label for RNA
Author: Gophane, Dnyaneshwar B.
Endeward, Burkhard
Prisner, Thomas F.
Sigurdsson, Snorri   orcid.org/0000-0003-2492-1456
Date: 2018
Language: English
Scope: 816-824
University/Institute: Háskóli Íslands
University of Iceland
School: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Department: Raunvísindadeild (HÍ)
Faculty of Physical Sciences (UI)
Raunvísindastofnun (HÍ)
Science Institute (UI)
Series: Organic & Biomolecular Chemistry;16(5)
ISSN: 1477-0520
1477-0539 (eISSN)
DOI: 10.1039/C7OB02870A
Subject: DNA-rannsóknir; Kjarnsýrur; Rafeindir
URI: https://hdl.handle.net/20.500.11815/1962

Show full item record


Gophane, D. B., Endeward, B., Prisner, T. F., & Sigurdsson, S. T. (2018). A semi-rigid isoindoline-derived nitroxide spin label for RNA. Organic and Biomolecular Chemistry, 16(5), 816-824 doi: 10.1039/c7ob02870a


A new isoindoline-derived benzimidazole nitroxide spin label, ImUm, was synthesized and incorporated into RNA oligoribonucleotides. ImUm is the first example of a conformationally unambiguous spin label for RNA, in which the nitroxide N–O bond lies on the same axis as the single bond used to attach the rigid isoindoline-based spin label to a uridine base. This results in minimal displacement of the nitroxide upon rotation of this single bond, which is a useful property for a label to be used for distance measurements. Continuous-wave (CW) EPR measurements of RNA duplexes containing ImUm indicate a restricted rotation around this single bond, presumably due to an intramolecular hydrogen bond between the benzimidazole N–H and O4 of the uracil. Orientation-selective pulsed electron–electron double resonance (PELDOR, also called double electron–electron resonance, or DEER) distance measurements between two spin labels in two RNA duplexes showed in one case a strong orientation dependence, further confirming the restricted motion of the spin labels in RNA duplexes.


Post-print (lokagerð höfundar)

Files in this item

This item appears in the following Collection(s)