bcTol: a highly water-soluble biradical for efficient dynamic nuclear polarization of biomolecules

Jagtap, Anil P.; Geiger, Michel-Andreas; Stöppler, Daniel; Orwick-Rydmark, Marcella; Oschkinat, Hartmut; Sigurdsson, Snorri

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dc.contributor	Háskóli Íslands
dc.contributor	University of Iceland
dc.contributor.author	Jagtap, Anil P.
dc.contributor.author	Geiger, Michel-Andreas
dc.contributor.author	Stöppler, Daniel
dc.contributor.author	Orwick-Rydmark, Marcella
dc.contributor.author	Oschkinat, Hartmut
dc.contributor.author	orcid.org/0000-0003-2492-1456 Sigurdsson, Snorri
dc.date.accessioned	2020-08-10T15:20:15Z
dc.date.available	2020-08-10T15:20:15Z
dc.date.issued	2016
dc.identifier.citation	Jagtap, A. P., Geiger, M. -A., Stöppler, D., Orwick-Rydmark, M., Oschkinat, H., & Sigurdsson, S. T. (2016). BcTol: A highly water-soluble biradical for efficient dynamic nuclear polarization of biomolecules. Chemical Communications, 52(43), 7020-7023. doi:10.1039/c6cc01813k
dc.identifier.issn	1359-7345
dc.identifier.issn	1364-548X (eISSN)
dc.identifier.uri	https://hdl.handle.net/20.500.11815/1959
dc.description	Post-print (lokagerð höfundar)
dc.description.abstract	Dynamic nuclear polarization (DNP) is an efficient method to overcome the inherent low sensitivity of magic-angle spinning (MAS) solid-state NMR. We report a new polarizing agent (bcTol), designed for biological applications, that yielded an enhancement value of 244 in a microcrystalline SH3 domain sample at 110 K.
dc.description.sponsorship	This work was financially supported by the Icelandic Research Fund (141062051), the Deutsche Forschungsgemeinschaft (SFB 1078, 740 and 765) and by a doctoral fellowship to A. P. J. from the University of Icelandic Research Fund. We thank A. Diehl, K. Rehbein, N. Erdmann and D. Michl for the preparation of microcrystalline SH3 and channelrhodopsin samples, Dr S. Jonsdottir for assistance in collecting analytical data for structural characterization of the radicals, as well as P. Hegemann and K. Stehfest for helpful discussions concerning expression and purification of channelrhodopsin.
dc.format.extent	7020-7023
dc.language.iso	en
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation.ispartofseries	Chemical Communications;52(43)
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Angle spinning NMR
dc.subject	Solid state
dc.subject	Nitroxide biradicals
dc.subject	Magnetic resonance
dc.subject	Amyloid fibrils
dc.subject	Protein
dc.subject	Resolution
dc.subject	Spectroscopy
dc.subject	Agents
dc.subject	Litrófsgreining
dc.subject	Segulmagn
dc.subject	Prótín
dc.subject	Kjarnsýrur
dc.title	bcTol: a highly water-soluble biradical for efficient dynamic nuclear polarization of biomolecules
dc.type	info:eu-repo/semantics/article
dc.description.version	Peer reviewed
dc.identifier.journal	Chemical Communications
dc.identifier.doi	10.1039/C6CC01813K
dc.relation.url	http://pubs.rsc.org/en/content/articlepdf/2016/CC/C6CC01813K
dc.contributor.department	Raunvísindadeild (HÍ)
dc.contributor.department	Faculty of Physical Sciences (UI)
dc.contributor.department	Raunvísindastofnun (HÍ)
dc.contributor.department	Science Institute (UI)
dc.contributor.school	Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school	School of Engineering and Natural Sciences (UI)