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Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA
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| Title: | Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA |
| Author: |
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| Date: | 2015 |
| Language: | English |
| Scope: | 15098-15102 |
| University/Institute: | Háskóli Íslands University of Iceland |
| School: | Verkfræði- og náttúruvísindasvið (HÍ) School of Engineering and Natural Sciences (UI) |
| Department: | Raunvísindadeild (HÍ) Faculty of Physical Sciences (UI) Raunvísindastofnun (HÍ) Science Institute (UI) |
| Series: | Physical Chemistry Chemical Physics;17(23) |
| ISSN: | 1463-9076 1463-9084 (eISSN) |
| DOI: | 10.1039/C5CP01624J |
| Subject: | Metal ion binding; Electronic double resonance; Dipolar spectroscopy; Hammerhead ribozyme; ESR spectroscopy; Protein dimer; Probe; Litrófsgreining; Kjarnsýrur; Rafeindir |
| URI: | https://hdl.handle.net/20.500.11815/1956 |
Citation:Kaminker, I., Bye, M., Mendelman, N., Gislason, K., Sigurdsson, S. T., & Goldfarb, D. (2015). Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA. Physical Chemistry Chemical Physics, 17(23), 15098-15102 doi:10.1039/c5cp01624j
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Abstract:Mn2+ localization in hairpin 92 of the 23S ribosomal RNA (HP92) was obtained using W-band (95 GHz) DEER (double electron-electron resonance) distance measurements between the Mn2+ ion and nitroxide spin labels on the RNA. It was found to be preferably situated in the minor groove of the double strand region close to the HP92 loop.
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Description:Post-print (lokagerð höfundar)
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