Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA
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Höfundar
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Útgefandi
Royal Society of Chemistry (RSC)
Úrdráttur
Mn2+ localization in hairpin 92 of the 23S ribosomal RNA (HP92) was obtained using W-band (95 GHz) DEER (double electron-electron resonance) distance measurements between the Mn2+ ion and nitroxide spin labels on the RNA. It was found to be preferably situated in the minor groove of the double strand region close to the HP92 loop.
Lýsing
Post-print (lokagerð höfundar)
Efnisorð
Metal ion binding, Electronic double resonance, Dipolar spectroscopy, Hammerhead ribozyme, ESR spectroscopy, Protein dimer, Probe, Litrófsgreining, Kjarnsýrur, Rafeindir
Citation
Kaminker, I., Bye, M., Mendelman, N., Gislason, K., Sigurdsson, S. T., & Goldfarb, D. (2015). Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA. Physical Chemistry Chemical Physics, 17(23), 15098-15102 doi:10.1039/c5cp01624j