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Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA

Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA


Titill: Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA
Höfundur: Kaminker, Ilia
Bye, Morgan
Mendelman, Natanel
Gislason, Kristmann
Sigurdsson, Snorri   orcid.org/0000-0003-2492-1456
Goldfarb, Daniella
Útgáfa: 2015
Tungumál: Enska
Umfang: 15098-15102
Háskóli/Stofnun: Háskóli Íslands
University of Iceland
Svið: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Deild: Raunvísindadeild (HÍ)
Faculty of Physical Sciences (UI)
Raunvísindastofnun (HÍ)
Science Institute (UI)
Birtist í: Physical Chemistry Chemical Physics;17(23)
ISSN: 1463-9076
1463-9084 (eISSN)
DOI: 10.1039/C5CP01624J
Efnisorð: Metal ion binding; Electronic double resonance; Dipolar spectroscopy; Hammerhead ribozyme; ESR spectroscopy; Protein dimer; Probe; Litrófsgreining; Kjarnsýrur; Rafeindir
URI: https://hdl.handle.net/20.500.11815/1956

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Tilvitnun:

Kaminker, I., Bye, M., Mendelman, N., Gislason, K., Sigurdsson, S. T., & Goldfarb, D. (2015). Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA. Physical Chemistry Chemical Physics, 17(23), 15098-15102 doi:10.1039/c5cp01624j

Útdráttur:

Mn2+ localization in hairpin 92 of the 23S ribosomal RNA (HP92) was obtained using W-band (95 GHz) DEER (double electron-electron resonance) distance measurements between the Mn2+ ion and nitroxide spin labels on the RNA. It was found to be preferably situated in the minor groove of the double strand region close to the HP92 loop.

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