Opin vísindi

Noncovalent Spin‐Labeling of DNA and RNA Triplexes

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Kamble, Nilesh Ramesh
dc.contributor.author Wolfrum, Manpreet
dc.contributor.author Halbritter, Thomas
dc.contributor.author Sigurdsson, Snorri
dc.contributor.author Richert, Clemens
dc.date.accessioned 2020-08-07T14:06:50Z
dc.date.available 2020-08-07T14:06:50Z
dc.date.issued 2020-01-21
dc.identifier.citation Kamble, N., Wolfrum, M., Halbritter, T., Sigurdsson, S. T., & Richert, C. (2020). Noncovalent Spin-Labeling of DNA and RNA Triplexes. 17(2), e1900676. doi:10.1002/cbdv.201900676
dc.identifier.issn 1612-1872
dc.identifier.issn 1612-1880 (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/1938
dc.description Publisher's version (útgefin grein)
dc.description.abstract Studying nucleic acids often requires labeling. Many labeling approaches require covalent bonds between the nucleic acid and the label, which complicates experimental procedures. Noncovalent labeling avoids the need for highly specific reagents and reaction conditions, and the effort of purifying bioconjugates. Among the least invasive techniques for studying biomacromolecules are NMR and EPR. Here, we report noncovalent labeling of DNA and RNA triplexes with spin labels that are nucleobase derivatives. Spectroscopic signals indicating strong binding were detected in EPR experiments in the cold, and filtration assays showed micromolar dissociation constants for complexes between a guanine‐derived label and triplex motifs containing a single‐nucleotide gap in the oligopurine strand. The advantages and challenges of noncovalent labeling via this approach that complements techniques relying on covalent links are discussed.
dc.description.sponsorship The authors thank Anna‐Lena J. Segler for technical assistance. This work was supported by the Icelandic Research Fund, grant No. 141062‐051 to S.T.S. and DFG, grant No. RI 1063/16‐1 to C.R. T.H. thanks the Deutsche Forschungsgemeinschaft (DFG) for a postdoctoral fellowship (414196920).
dc.format.extent e1900676
dc.language.iso en
dc.publisher Wiley
dc.relation.ispartofseries Chemistry & Biodiversity;17(2)
dc.rights info:eu-repo/semantics/openAccess
dc.subject DNA
dc.subject RNA
dc.subject Triplexes
dc.subject Spin labeling
dc.subject EPR
dc.subject Kjarnsýrur
dc.title Noncovalent Spin‐Labeling of DNA and RNA Triplexes
dc.type info:eu-repo/semantics/article
dcterms.license This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
dc.description.version Peer reviewed
dc.identifier.journal Chemistry & Biodiversity
dc.identifier.doi 10.1002/cbdv.201900676
dc.contributor.department Raunvísindadeild (HÍ)
dc.contributor.department Faculty of Physical Sciences (UI)
dc.contributor.department Raunvísindastofnun (HÍ)
dc.contributor.department Science Institute (UI)
dc.contributor.school Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school School of Engineering and Natural Sciences (UI)

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