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Noncovalent Spin‐Labeling of DNA and RNA Triplexes

Noncovalent Spin‐Labeling of DNA and RNA Triplexes

Title: Noncovalent Spin‐Labeling of DNA and RNA Triplexes
Author: Kamble, Nilesh Ramesh
Wolfrum, Manpreet
Halbritter, Thomas   orcid.org/0000-0002-4642-0049
Sigurdsson, Snorri   orcid.org/0000-0003-2492-1456
Richert, Clemens
Date: 2020-01-21
Language: English
Scope: e1900676
University/Institute: Háskóli Íslands
University of Iceland
School: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Department: Raunvísindadeild (HÍ)
Faculty of Physical Sciences (UI)
Raunvísindastofnun (HÍ)
Science Institute (UI)
Series: Chemistry & Biodiversity;17(2)
ISSN: 1612-1872
1612-1880 (eISSN)
DOI: 10.1002/cbdv.201900676
Subject: DNA; RNA; Triplexes; Spin labeling; EPR; Kjarnsýrur
URI: https://hdl.handle.net/20.500.11815/1938

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Kamble, N., Wolfrum, M., Halbritter, T., Sigurdsson, S. T., & Richert, C. (2020). Noncovalent Spin-Labeling of DNA and RNA Triplexes. 17(2), e1900676. doi:10.1002/cbdv.201900676


Studying nucleic acids often requires labeling. Many labeling approaches require covalent bonds between the nucleic acid and the label, which complicates experimental procedures. Noncovalent labeling avoids the need for highly specific reagents and reaction conditions, and the effort of purifying bioconjugates. Among the least invasive techniques for studying biomacromolecules are NMR and EPR. Here, we report noncovalent labeling of DNA and RNA triplexes with spin labels that are nucleobase derivatives. Spectroscopic signals indicating strong binding were detected in EPR experiments in the cold, and filtration assays showed micromolar dissociation constants for complexes between a guanine‐derived label and triplex motifs containing a single‐nucleotide gap in the oligopurine strand. The advantages and challenges of noncovalent labeling via this approach that complements techniques relying on covalent links are discussed.


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