Opin vísindi

Lipidomic study of cell lines reveals differences between breast cancer subtypes

Lipidomic study of cell lines reveals differences between breast cancer subtypes

Title: Lipidomic study of cell lines reveals differences between breast cancer subtypes
Author: Eiríksson, Finnur F.
Nøhr, Martha Kampp
Costa, Margarida   orcid.org/0000-0001-9147-8621
Bodvarsdottir, Sigridur Klara   orcid.org/0000-0001-8799-8018
Ögmundsdóttir, Helga M.
Þorsteinsdóttir, Margrét
Date: 2020-04-14
Language: English
Scope: e0231289
University/Institute: Háskóli Íslands
University of Iceland
School: Heilbrigðisvísindasvið (HÍ)
School of Health Sciences (UI)
Department: Lyfjafræðideild (HÍ)
Faculty of Pharmaceutical Sciences (UI)
Læknadeild (HÍ)
Faculty of Medicine (UI)
Series: Plos One;15(4)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0231289
Subject: Lipids; Breast cancer; Lipid analysis; Lipid metabolism; Principal component analysis; Cancer treatment; Computer software; Transcriptome analysis; Brjóstakrabbamein; Fituefni; Krabbameinslækningar; Hugbúnaður
URI: https://hdl.handle.net/20.500.11815/1921

Show full item record


Eiriksson FF, Nøhr MK, Costa M, Bödvarsdottir SK, Ögmundsdottir HM, Thorsteinsdottir M (2020) Lipidomic study of cell lines reveals differences between breast cancer subtypes. PLoS ONE 15(4): e0231289. https://doi.org/10.1371/journal.pone.0231289


Breast cancer (BC) is the most prevalent type of cancer in women in western countries. BC mortality has not declined despite early detection by screening, indicating the need for better informed treatment decisions. Therefore, a novel noninvasive diagnostic tool for BC would give the opportunity of subtype-specific treatment and improved prospects for the patients. Heterogeneity of BC tumor subtypes is reflected in the expression levels of enzymes in lipid metabolism. The aim of the study was to investigate whether the subtype defined by the transcriptome is reflected in the lipidome of BC cell lines. A liquid chromatography mass spectrometry (LC-MS) platform was applied to analyze the lipidome of six cell lines derived from human BC cell lines representing different BC subtypes. We identified an increased abundance of triacylglycerols (TG) ≥ C-48 with moderate or multiple unsaturation in fatty acyl chains and down-regulated ether-phosphatidylethanolamines (PE) (C-34 to C-38) in cell lines representing estrogen receptor and progesterone receptor positive tumor subtypes. In a cell line representing HER2-overexpressing tumor subtype an elevated expression of TG (≤ C-46), phosphatidylcholines (PC) and PE containing short-chained (≤ C-16) saturated or monounsaturated fatty acids were observed. Increased abundance of PC ≥ C-40 was found in cell lines of triple negative BC subtype. In addition, differences were detected in lipidomes within these previously defined subtypes. We conclude that subtypes defined by the transcriptome are indeed reflected in differences in the lipidome and, furthermore, potentially biologically relevant differences may exist within these defined subtypes.


Publisher's version (útgefin grein)


This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Files in this item

This item appears in the following Collection(s)