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Lipidomic study of cell lines reveals differences between breast cancer subtypes

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Eiríksson, Finnur F.
dc.contributor.author Nøhr, Martha Kampp
dc.contributor.author Costa, Margarida
dc.contributor.author Bodvarsdottir, Sigridur Klara
dc.contributor.author Ögmundsdóttir, Helga M.
dc.contributor.author Þorsteinsdóttir, Margrét
dc.date.accessioned 2020-08-05T15:28:52Z
dc.date.available 2020-08-05T15:28:52Z
dc.date.issued 2020-04-14
dc.identifier.citation Eiriksson FF, Nøhr MK, Costa M, Bödvarsdottir SK, Ögmundsdottir HM, Thorsteinsdottir M (2020) Lipidomic study of cell lines reveals differences between breast cancer subtypes. PLoS ONE 15(4): e0231289. https://doi.org/10.1371/journal.pone.0231289
dc.identifier.issn 1932-6203
dc.identifier.uri https://hdl.handle.net/20.500.11815/1921
dc.description Publisher's version (útgefin grein)
dc.description.abstract Breast cancer (BC) is the most prevalent type of cancer in women in western countries. BC mortality has not declined despite early detection by screening, indicating the need for better informed treatment decisions. Therefore, a novel noninvasive diagnostic tool for BC would give the opportunity of subtype-specific treatment and improved prospects for the patients. Heterogeneity of BC tumor subtypes is reflected in the expression levels of enzymes in lipid metabolism. The aim of the study was to investigate whether the subtype defined by the transcriptome is reflected in the lipidome of BC cell lines. A liquid chromatography mass spectrometry (LC-MS) platform was applied to analyze the lipidome of six cell lines derived from human BC cell lines representing different BC subtypes. We identified an increased abundance of triacylglycerols (TG) ≥ C-48 with moderate or multiple unsaturation in fatty acyl chains and down-regulated ether-phosphatidylethanolamines (PE) (C-34 to C-38) in cell lines representing estrogen receptor and progesterone receptor positive tumor subtypes. In a cell line representing HER2-overexpressing tumor subtype an elevated expression of TG (≤ C-46), phosphatidylcholines (PC) and PE containing short-chained (≤ C-16) saturated or monounsaturated fatty acids were observed. Increased abundance of PC ≥ C-40 was found in cell lines of triple negative BC subtype. In addition, differences were detected in lipidomes within these previously defined subtypes. We conclude that subtypes defined by the transcriptome are indeed reflected in differences in the lipidome and, furthermore, potentially biologically relevant differences may exist within these defined subtypes.
dc.description.sponsorship This work was supported by the Icelandic Centre for Research, Reykjavik, Iceland (project grant no. 174566051) and the University of Iceland Research Fund, Reykjavik, Iceland. The funding supplied the salary for MKN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.format.extent e0231289
dc.language.iso en
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartofseries Plos One;15(4)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Lipids
dc.subject Breast cancer
dc.subject Lipid analysis
dc.subject Lipid metabolism
dc.subject Principal component analysis
dc.subject Cancer treatment
dc.subject Computer software
dc.subject Transcriptome analysis
dc.subject Brjóstakrabbamein
dc.subject Fituefni
dc.subject Krabbameinslækningar
dc.subject Hugbúnaður
dc.title Lipidomic study of cell lines reveals differences between breast cancer subtypes
dc.type info:eu-repo/semantics/article
dcterms.license This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.version Peer Reviewed
dc.identifier.journal Plos One
dc.identifier.doi 10.1371/journal.pone.0231289
dc.relation.url https://dx.plos.org/10.1371/journal.pone.0231289
dc.contributor.department Lyfjafræðideild (HÍ)
dc.contributor.department Faculty of Pharmaceutical Sciences (UI)
dc.contributor.department Læknadeild (HÍ)
dc.contributor.department Faculty of Medicine (UI)
dc.contributor.school Heilbrigðisvísindasvið (HÍ)
dc.contributor.school School of Health Sciences (UI)

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