dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Eiríksson, Finnur F. |
dc.contributor.author |
Nøhr, Martha Kampp |
dc.contributor.author |
Costa, Margarida |
dc.contributor.author |
Bodvarsdottir, Sigridur Klara |
dc.contributor.author |
Ögmundsdóttir, Helga M. |
dc.contributor.author |
Þorsteinsdóttir, Margrét |
dc.date.accessioned |
2020-08-05T15:28:52Z |
dc.date.available |
2020-08-05T15:28:52Z |
dc.date.issued |
2020-04-14 |
dc.identifier.citation |
Eiriksson FF, Nøhr MK, Costa M, Bödvarsdottir SK, Ögmundsdottir HM, Thorsteinsdottir M (2020) Lipidomic study of cell lines reveals differences between breast cancer subtypes. PLoS ONE 15(4): e0231289. https://doi.org/10.1371/journal.pone.0231289 |
dc.identifier.issn |
1932-6203 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1921 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
Breast cancer (BC) is the most prevalent type of cancer in women in western countries. BC mortality has not declined despite early detection by screening, indicating the need for better informed treatment decisions. Therefore, a novel noninvasive diagnostic tool for BC would give the opportunity of subtype-specific treatment and improved prospects for the patients. Heterogeneity of BC tumor subtypes is reflected in the expression levels of enzymes in lipid metabolism. The aim of the study was to investigate whether the subtype defined by the transcriptome is reflected in the lipidome of BC cell lines. A liquid chromatography mass spectrometry (LC-MS) platform was applied to analyze the lipidome of six cell lines derived from human BC cell lines representing different BC subtypes. We identified an increased abundance of triacylglycerols (TG) ≥ C-48 with moderate or multiple unsaturation in fatty acyl chains and down-regulated ether-phosphatidylethanolamines (PE) (C-34 to C-38) in cell lines representing estrogen receptor and progesterone receptor positive tumor subtypes. In a cell line representing HER2-overexpressing tumor subtype an elevated expression of TG (≤ C-46), phosphatidylcholines (PC) and PE containing short-chained (≤ C-16) saturated or monounsaturated fatty acids were observed. Increased abundance of PC ≥ C-40 was found in cell lines of triple negative BC subtype. In addition, differences were detected in lipidomes within these previously defined subtypes. We conclude that subtypes defined by the transcriptome are indeed reflected in differences in the lipidome and, furthermore, potentially biologically relevant differences may exist within these defined subtypes. |
dc.description.sponsorship |
This work was supported by the Icelandic Centre for Research, Reykjavik, Iceland (project grant no. 174566051) and the University of Iceland Research Fund, Reykjavik, Iceland. The funding supplied the salary for MKN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
dc.format.extent |
e0231289 |
dc.language.iso |
en |
dc.publisher |
Public Library of Science (PLoS) |
dc.relation.ispartofseries |
Plos One;15(4) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Lipids |
dc.subject |
Breast cancer |
dc.subject |
Lipid analysis |
dc.subject |
Lipid metabolism |
dc.subject |
Principal component analysis |
dc.subject |
Cancer treatment |
dc.subject |
Computer software |
dc.subject |
Transcriptome analysis |
dc.subject |
Brjóstakrabbamein |
dc.subject |
Fituefni |
dc.subject |
Krabbameinslækningar |
dc.subject |
Hugbúnaður |
dc.title |
Lipidomic study of cell lines reveals differences between breast cancer subtypes |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
Plos One |
dc.identifier.doi |
10.1371/journal.pone.0231289 |
dc.relation.url |
https://dx.plos.org/10.1371/journal.pone.0231289 |
dc.contributor.department |
Lyfjafræðideild (HÍ) |
dc.contributor.department |
Faculty of Pharmaceutical Sciences (UI) |
dc.contributor.department |
Læknadeild (HÍ) |
dc.contributor.department |
Faculty of Medicine (UI) |
dc.contributor.school |
Heilbrigðisvísindasvið (HÍ) |
dc.contributor.school |
School of Health Sciences (UI) |