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Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Knobloch, Stephen
dc.contributor.author Jóhannsson, Ragnar
dc.contributor.author Marteinsson, Viggó
dc.date.accessioned 2020-02-28T15:37:53Z
dc.date.available 2020-02-28T15:37:53Z
dc.date.issued 2019-07-18
dc.identifier.citation Knobloch, S., Jóhannsson, R. & Marteinsson, V. Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms. Scientific Reports 9, 10403 (2019). https://doi.org/10.1038/s41598-019-46904-3
dc.identifier.issn 2045-2322
dc.identifier.uri https://hdl.handle.net/20.500.11815/1561
dc.description Publisher's version (útgefin grein).
dc.description.abstract Marine sponges host bacterial symbionts with biotechnological potential, yet isolation of true sponge symbionts remains difficult due to their host dependency. Moreover, attempts to grow sponges for their pharmacologically-active compounds outside of their habitat often results in a shift of their microbial community. In this study we evaluate suitable sponge cultivation methods that allow maintenance of both the marine sponge Halichondria panicea and its associated bacteria in an ex situ environment. In addition, we present a method for co-cultivation of sponge explants and microbes separated by a membrane in a multi-chamber device. Tests on ex situ cultivation of H. panicea under different controlled conditions showed that only high water exchange rates in the aquarium enabled maintenance of its dominant symbiont “Candidatus Halichondribacter symbioticus” at a high relative abundance in the sponge body, a prerequisite for co-cultivation. The bacterial enrichment retrieved from co-cultivation contained bacteria from nine different classes in addition to sequences corresponding to “Ca. H. symbioticus”. This represents an increase of the cultivable bacterial classes from H. panicea compared to standard isolation techniques on solid media plates. The current study provides insights into sponge-microbe maintenance under ex situ conditions and proposes a new method for the isolation of sponge-associated bacteria.
dc.description.sponsorship We thank Ásgeir Guðnason and Þorkell Heiðarsson for providing facilities and help with the cultivation experiments. This work was supported by the EC Marie Curie Actions ITN Project BluePharmTrain [No. 607786].
dc.format.extent 10403
dc.language.iso en
dc.publisher Springer Science and Business Media LLC
dc.relation.ispartofseries Scientific Reports;9(1)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Applied microbiology
dc.subject Environmental microbiology
dc.subject Örverufræði
dc.subject Svampdýr
dc.title Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms
dc.type info:eu-repo/semantics/article
dcterms.license Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.description.version Peer Reviewed
dc.identifier.journal Scientific Reports
dc.identifier.doi 10.1038/s41598-019-46904-3
dc.contributor.department Líf- og umhverfisvísindadeild (HÍ)
dc.contributor.department Faculty of Life and Environmental Sciences (UI)
dc.contributor.school Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school School of Engineering and Natural Sciences (UI)

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