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Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms

Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms


Title: Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms
Author: Knobloch, Stephen   orcid.org/0000-0002-0930-8592
Jóhannsson, Ragnar
Marteinsson, Viggó   orcid.org/0000-0001-8340-821X
Date: 2019-07-18
Language: English
Scope: 10403
University/Institute: Háskóli Íslands
University of Iceland
School: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Department: Líf- og umhverfisvísindadeild (HÍ)
Faculty of Life and Environmental Sciences (UI)
Series: Scientific Reports;9(1)
ISSN: 2045-2322
DOI: 10.1038/s41598-019-46904-3
Subject: Applied microbiology; Environmental microbiology; Örverufræði; Svampdýr
URI: https://hdl.handle.net/20.500.11815/1561

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Citation:

Knobloch, S., Jóhannsson, R. & Marteinsson, V. Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms. Scientific Reports 9, 10403 (2019). https://doi.org/10.1038/s41598-019-46904-3

Abstract:

Marine sponges host bacterial symbionts with biotechnological potential, yet isolation of true sponge symbionts remains difficult due to their host dependency. Moreover, attempts to grow sponges for their pharmacologically-active compounds outside of their habitat often results in a shift of their microbial community. In this study we evaluate suitable sponge cultivation methods that allow maintenance of both the marine sponge Halichondria panicea and its associated bacteria in an ex situ environment. In addition, we present a method for co-cultivation of sponge explants and microbes separated by a membrane in a multi-chamber device. Tests on ex situ cultivation of H. panicea under different controlled conditions showed that only high water exchange rates in the aquarium enabled maintenance of its dominant symbiont “Candidatus Halichondribacter symbioticus” at a high relative abundance in the sponge body, a prerequisite for co-cultivation. The bacterial enrichment retrieved from co-cultivation contained bacteria from nine different classes in addition to sequences corresponding to “Ca. H. symbioticus”. This represents an increase of the cultivable bacterial classes from H. panicea compared to standard isolation techniques on solid media plates. The current study provides insights into sponge-microbe maintenance under ex situ conditions and proposes a new method for the isolation of sponge-associated bacteria.

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Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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