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Northern lights assay: a versatile method for comprehensive detection of DNA damage

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Guðmundsson, Bjarki
dc.contributor.author Þormar, Hans G
dc.contributor.author Sigurðsson, Albert
dc.contributor.author Dankers, Wendy
dc.contributor.author Steinarsdóttir, Margrét
dc.contributor.author Hermanowicz, Stefan
dc.contributor.author Sigurðsson, Stefán
dc.contributor.author Ólafsson, Davið
dc.contributor.author Halldorsdottir, Anna
dc.contributor.author Meyn, Stephen
dc.contributor.author Jonsson, Jon J.
dc.date.accessioned 2019-12-05T15:18:24Z
dc.date.available 2019-12-05T15:18:24Z
dc.date.issued 2018-07-24
dc.identifier.citation Bjarki Gudmundsson, Hans G Thormar, Albert Sigurdsson, Wendy Dankers, Margret Steinarsdottir, Stefan Hermanowicz, Stefan Sigurdsson, David Olafsson, Anna M Halldorsdottir, Stephen Meyn, Jon J Jonsson, Northern lights assay: a versatile method for comprehensive detection of DNA damage, Nucleic Acids Research, Volume 46, Issue 20, 16 November 2018, Page e118, https://doi.org/10.1093/nar/gky645
dc.identifier.issn 0305-1048
dc.identifier.issn 1362-4962 (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/1373
dc.description Publisher's version (útgefin grein)
dc.description.abstract DNA damage assays have various limitations in types of lesions detected, sensitivity, specificity and samples that can be analyzed. The Northern Lights Assay (NLA) is based on 2D Strandness-Dependent Electrophoresis (2D-SDE), a technique that separates nucleic acids based on length, strandness, structure and conformation changes induced by damage. NLA is run on a microgel platform in 20- 25 min. Each specimen is analyzed in pairs of nondigested DNA to detect single- and double-stranded breaks (DSBs) and Mbo I-digested DNA to detect other lesions. We used NLA to evaluate DNA in solution and isolated from human cells treated with various genotoxic agents. NLA detected and distinguished between single- and DSBs, interstrand and intrastrand DNA crosslinks, and denatured singlestranded DNA. NLA was sufficiently sensitive to detect biologically relevant amount of DNA damage. NLA is a versatile, sensitive and simple method for comprehensive and simultaneous analysis of multiple types of damage, both in purified DNA and in DNA isolated from cells and body fluids. NLA can be used to evaluate DNA quality in biosamples, monitor complex molecular procedures, assess genotoxicity, diagnose genome instability, facilitate cancer theranostics and in basic nucleic acids research.
dc.description.sponsorship We wish to thank Agata Smogorzewska and Supawat Thongthip (The Laboratory of Genome Maintenance, Rockefeller University, New York, USA), for the kind gift of FA cell lines and control cell lines. University of Iceland Research Fund [210757-5389]; Landspitali University Hospital Research Fund; Icelandic Center for Research Funds [1427090611]; L´ıfeind ehf. (to B.G) (in part), (to H.G.Th.). Funding for open access charge: University of Iceland Research Fund. Conflict of interest statement. B.G., H.G.Th. and J.J.J. are stockholders in Lifeind ehf., a company that holds the patent to 2D-SDE and is marketing the NLA. J.J.J. receives research funding from Lifeind ehf. and H.G.Th. is employed by Lifeind ehf.
dc.format.extent e118
dc.language.iso en
dc.publisher Oxford University Press (OUP)
dc.relation.ispartofseries Nucleic Acids Research;46(20)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Genetics
dc.subject Cell DNA
dc.subject Genomic DNA
dc.subject Nucleic acid
dc.subject Erfðafræði
dc.subject DNA-rannsóknir
dc.title Northern lights assay: a versatile method for comprehensive detection of DNA damage
dc.type info:eu-repo/semantics/article
dcterms.license This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.description.version Peer reviewed
dc.identifier.journal Nucleic Acids Research
dc.identifier.doi 10.1093/nar/gky645
dc.relation.url http://academic.oup.com/nar/advance-article-pdf/doi/10.1093/nar/gky645/25204881/gky645.pdf
dc.contributor.department Faculty of Medicine (UI)
dc.contributor.department The Biomedical Center (BMC) (UI)
dc.contributor.department Lífvísindasetur (HÍ)
dc.contributor.department Læknadeild (HÍ)
dc.contributor.school Heilbrigðisvísindasvið (HÍ)
dc.contributor.school School of Health Sciences (UI)

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