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Northern lights assay: a versatile method for comprehensive detection of DNA damage

Northern lights assay: a versatile method for comprehensive detection of DNA damage


Title: Northern lights assay: a versatile method for comprehensive detection of DNA damage
Author: Guðmundsson, Bjarki
Þormar, Hans G
Sigurðsson, Albert
Dankers, Wendy   orcid.org/0000-0002-4180-0472
Steinarsdóttir, Margrét
Hermanowicz, Stefan
Sigurðsson, Stefán
Ólafsson, Davið
Halldorsdottir, Anna   orcid.org/0000-0002-3800-1223
Meyn, Stephen
... 1 more authors Show all authors
Date: 2018-07-24
Language: English
Scope: e118
University/Institute: Háskóli Íslands (HÍ)
University of Iceland (UI)
School: Heilbrigðisvísindasvið (HÍ)
School of Health Sciences (UI)
Department: Faculty of Medicine (UI)
The Biomedical Center (BMC) (UI)
Lífvísindasetur (HÍ)
Læknadeild (HÍ)
Series: Nucleic Acids Research;46(20)
ISSN: 0305-1048
1362-4962 (eISSN)
DOI: 10.1093/nar/gky645
Subject: Genetics; Cell DNA; Genomic DNA; Nucleic acid; Erfðafræði; DNA-rannsóknir
URI: https://hdl.handle.net/20.500.11815/1373

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Citation:

Bjarki Gudmundsson, Hans G Thormar, Albert Sigurdsson, Wendy Dankers, Margret Steinarsdottir, Stefan Hermanowicz, Stefan Sigurdsson, David Olafsson, Anna M Halldorsdottir, Stephen Meyn, Jon J Jonsson, Northern lights assay: a versatile method for comprehensive detection of DNA damage, Nucleic Acids Research, Volume 46, Issue 20, 16 November 2018, Page e118, https://doi.org/10.1093/nar/gky645

Abstract:

DNA damage assays have various limitations in types of lesions detected, sensitivity, specificity and samples that can be analyzed. The Northern Lights Assay (NLA) is based on 2D Strandness-Dependent Electrophoresis (2D-SDE), a technique that separates nucleic acids based on length, strandness, structure and conformation changes induced by damage. NLA is run on a microgel platform in 20- 25 min. Each specimen is analyzed in pairs of nondigested DNA to detect single- and double-stranded breaks (DSBs) and Mbo I-digested DNA to detect other lesions. We used NLA to evaluate DNA in solution and isolated from human cells treated with various genotoxic agents. NLA detected and distinguished between single- and DSBs, interstrand and intrastrand DNA crosslinks, and denatured singlestranded DNA. NLA was sufficiently sensitive to detect biologically relevant amount of DNA damage. NLA is a versatile, sensitive and simple method for comprehensive and simultaneous analysis of multiple types of damage, both in purified DNA and in DNA isolated from cells and body fluids. NLA can be used to evaluate DNA quality in biosamples, monitor complex molecular procedures, assess genotoxicity, diagnose genome instability, facilitate cancer theranostics and in basic nucleic acids research.

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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