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Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Allahgholi, Leila
dc.contributor.author Sardari, Roya R. R.
dc.contributor.author Hakvåg, Sigrid
dc.contributor.author Ara, Kazi Z. G.
dc.contributor.author Kristjánsdóttir, Þórdís
dc.contributor.author Aasen, Inga Marie
dc.contributor.author Friðjónsson, Ólafur H.
dc.contributor.author Brautaset, Trygve
dc.contributor.author Hreggvidsson, Gudmundur Oli
dc.contributor.author Karlsson, Eva Nordberg
dc.date.accessioned 2021-01-11T14:16:07Z
dc.date.available 2021-01-11T14:16:07Z
dc.date.issued 2020-03-30
dc.identifier.citation Allahgholi, L., Sardari, R.R.R., Hakvåg, S. et al. Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles. Journal of Applied Phycology 32, 1933–1947 (2020). https://doi.org/10.1007/s10811-020-02103-6
dc.identifier.issn 0921-8971
dc.identifier.issn 1573-5176 (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/2344
dc.description Publisher's version (útgefin grein)
dc.description.abstract Brown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining and several processing problems need to be solved to make them a viable option. Pre-treatment is necessary to enhance accessibility and solubility of the biomass components but should be minimal and mild to assure sustainable and cost-effective processing. Here, two routes to pre-treatLaminaria digitata to release polysaccharides were investigated: hot water pre-treatment by autoclaving (121 °C, 20 min or 60 min) and a two-step extraction with mild acid (0.1 M HCl) followed by alkaline treatment. Hot water pre-treatment resulted in partial extraction of a mixture of polysaccharides consisting of alginate, fucoidan and laminarin. After mild acid pre-treatment, alginate was found in the remaining insoluble residues and was extracted in a second step via alkaline treatment using Na2CO3 (0.15 M) at 80 °C and CaCl2 (10%) for the precipitation. In addition to carbohydrates, a fraction of other components such as proteins, phenolic compounds, minerals and trace elements was detected in the extracts. Cultivation of the thermophilic bacterial strains Rhodothermus marinus DSM 16675 and Bacillus methanolicus MGA3 (ATCC 53907) in media supplemented with the respective extracts resulted in growth of both strains, indicating that they were able to utilize the available carbon source for growth. R. marinus displayed the highest cell density in the medium containing the extract from acid pre-treatment, whereas B. methanolicus growth was highest with the extract from hot water pre-treatment.
dc.description.sponsorship Open access funding provided by Lund University. The authors received financial support for the projects Thermofactories, (Era-net MBT1, grant agreement No 604814), MacroVal (the Swedish research council Formas, grant 2015-769), Marine food resources for new markets (Formas, grant 2018-01863), Macro cascade (Bio-Based Industries Joint Undertaking under EU Horizon 2020, grant agreement No 720755), the Novo Nordisk foundation (grant agreement No NNF18OC00349792) and ProSeaFood, (Era-net SusFood2, grant agreement No 727473).
dc.format.extent 1933-1947
dc.language.iso en
dc.publisher Springer Science and Business Media LLC
dc.relation info:eu-repo/grantAgreement/EC/H2020/720755
dc.relation.ispartofseries Journal of Applied Phycology;32(3)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Bacillus methanolicus
dc.subject Carbohydrate extraction
dc.subject Laminaria digitata
dc.subject Mild physicochemical pre-treatment
dc.subject Rhodothermus marinus
dc.subject Hrossaþari
dc.subject Brúnþörungar
dc.title Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles
dc.type info:eu-repo/semantics/article
dcterms.license Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.description.version Peer Reviewed
dc.identifier.journal Journal of Applied Phycology
dc.identifier.doi 10.1007/s10811-020-02103-6
dc.relation.url https://link.springer.com/article/10.1007%2Fs10811-020-02103-6

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