dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Månberger, Anna |
dc.contributor.author |
Verbrugghe, Phebe |
dc.contributor.author |
Guðmundsdóttir, Elísabet Eik |
dc.contributor.author |
Santesson, Sara |
dc.contributor.author |
Nilsson, Anne |
dc.contributor.author |
Hreggvidsson, Gudmundur Oli |
dc.contributor.author |
Linares-Pastén, Javier A. |
dc.contributor.author |
Nordberg Karlsson, Eva |
dc.date.accessioned |
2020-10-30T15:13:47Z |
dc.date.available |
2020-10-30T15:13:47Z |
dc.date.issued |
2020-04-03 |
dc.identifier.citation |
Månberger, A., Verbrugghe, P., Guðmundsdóttir, E.E. et al. Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres. Sci Rep 10, 5853 (2020). https://doi.org/10.1038/s41598-020-62610-x |
dc.identifier.issn |
2045-2322 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/2153 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
The importance of the gut microbiota in human health has led to an increased interest to study probiotic bacteria. Fermented food is a source of already established probiotics, but it also offers an opportunity to discover new taxa. Four strains of Weissella sp. isolated from Indian fermented food have been genome sequenced and classified into the species W. cibaria based on whole-genome phylogeny. The genome of W. cibaria strain 92, known to utilise xylooligosaccharides and produce lactate and acetate, was analysed to identify genes for oligosaccharide utilisation. Clusters including genes involved in transportation, hydrolysis and metabolism of xylooligosaccharides, arabinooligosaccharides and β-glucosides were identified. Growth on arabinobiose and laminaribiose was detected. A 6-phospho-β-glucosidase clustered with a phosphotransferase system was found upregulated during growth on laminaribiose, indicating a mechanism for laminaribiose utilisation. The genome of W. cibaria strain 92 harbours genes for utilising the phosphoketolase pathway for the production of both acetate and lactate from pentose and hexose sugars but lacks two genes necessary for utilising the pentose phosphate pathway. The ability of W. cibaria strain 92 to utilise several types of oligosaccharides derived from dietary fibres, and produce lactate and acetate makes it interesting as a probiotic candidate for further evaluation. |
dc.description.sponsorship |
We gratefully thank the Swedish Research Council (VR), grant number 2014–05038 for the financial support to this study. Additional support of from the Macro Cascade project, with funding from the Bio-Based Industries Joint Undertaking under the European Union Horizon 2020 research and innovation programme under grant agreement No 720755 is gratefully acknowledged. Open access funding provided by Lund University. |
dc.format.extent |
5853 |
dc.language.iso |
en |
dc.publisher |
Springer Science and Business Media LLC |
dc.relation |
info:eu-repo/grantAgreement/EC/H2020/720755 |
dc.relation.ispartofseries |
Scientific Reports;10(1) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Probiotics |
dc.subject |
Fermented food |
dc.subject |
Taxogenomic assessment |
dc.subject |
Gerlar |
dc.subject |
Gerjun |
dc.subject |
Matvæli |
dc.subject |
Örverufræði |
dc.subject |
Erfðafræði |
dc.title |
Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
Scientific Reports |
dc.identifier.doi |
10.1038/s41598-020-62610-x |
dc.relation.url |
https://www.nature.com/articles/s41598-020-62610-x |
dc.contributor.department |
Líf- og umhverfisvísindadeild (HÍ) |
dc.contributor.department |
Faculty of Life and Environmental Sciences (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |