Opin vísindi

Engineering the carotenoid biosynthetic pathway in Rhodothermus marinus for lycopene production

Engineering the carotenoid biosynthetic pathway in Rhodothermus marinus for lycopene production


Title: Engineering the carotenoid biosynthetic pathway in Rhodothermus marinus for lycopene production
Author: Kristjánsdóttir, Þórdís   orcid.org/0000-0003-2244-1723
Ron, Emanuel Y.C.
Molins-Delgado, Daniel
Fridjonsson, Olafur   orcid.org/0000-0002-8725-602X
Turner, Charlotta
Björnsdóttir, Snædís   orcid.org/0000-0003-4368-8602
Guðmundsson, Steinn   orcid.org/0000-0002-2758-2720
van Niel, Ed W.J.
Karlsson, Eva Nordberg
Hreggvidsson, Gudmundur Oli   orcid.org/0000-0002-4958-1673
Date: 2020-12
Language: English
Scope: e00140
University/Institute: Háskóli Íslands
University of Iceland
School: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Department: Iðnaðarverkfræði-, vélaverkfræði- og tölvunarfræðideild (HÍ)
Faculty of Industrial Eng., Mechanical Eng. and Computer Science (UI)
Líf- og umhverfisvísindadeild (HÍ)
Faculty of Life and Environmental Sciences (UI)
Series: Metabolic Engineering Communications;11(1)
ISSN: 2214-0301
DOI: 10.1016/j.mec.2020.e00140
Subject: Carotenoids; Genetic engineering; Lycopene; Rhodothermus marinus; Erfðatækni; Gerlar
URI: https://hdl.handle.net/20.500.11815/2136

Show full item record

Citation:

Kristjansdottir, T., Ron, E. Y. C., Molins-Delgado, D., Fridjonsson, O. H., Turner, C., Bjornsdottir, S. H., . . . Hreggvidsson, G. O. (2020). Engineering the carotenoid biosynthetic pathway in rhodothermus marinus for lycopene production. Metabolic Engineering Communications, 11, 1. doi:http://dx.doi.org/10.1016/j.mec.2020.e00140

Abstract:

Rhodothermus marinus has the potential to be well suited for biorefineries, as an aerobic thermophile that produces thermostable enzymes and is able to utilize polysaccharides from different 2nd and 3rd generation biomass. The bacterium produces valuable chemicals such as carotenoids. However, the native carotenoids are not established for industrial production and R. marinus needs to be genetically modified to produce higher value carotenoids. Here we genetically modified the carotenoid biosynthetic gene cluster resulting in three different mutants, most importantly the lycopene producing mutant TK-3 (ΔtrpBΔpurAΔcruFcrtB::trpBcrtBT.thermophilus). The genetic modifications and subsequent structural analysis of carotenoids helped clarify the carotenoid biosynthetic pathway in R. marinus. The nucleotide sequences encoding the enzymes phytoene synthase (CrtB) and the previously unidentified 1′,2′-hydratase (CruF) were found fused together and encoded by a single gene in R. marinus. Deleting only the cruF part of the gene did not result in an active CrtB enzyme. However, by deleting the entire gene and inserting the crtB gene from Thermus thermophilus, a mutant strain was obtained, producing lycopene as the sole carotenoid. The lycopene produced by TK-3 was quantified as 0.49 ​g/kg CDW (cell dry weight).

Description:

Publisher's version (útgefin grein)

Rights:

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Files in this item

This item appears in the following Collection(s)