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Distribution of mast cells within the mouse heart and its dependency on Mitf

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Ingason, Arnar
dc.contributor.author Mechmet, Fatich
dc.contributor.author Atacho, Diahann
dc.contributor.author Steingrimsson, Eirikur
dc.contributor.author Petersen, Petur Henry
dc.date.accessioned 2020-10-14T15:23:02Z
dc.date.available 2020-10-14T15:23:02Z
dc.date.issued 2019-01
dc.identifier.citation Ingason, A., Mechmet, F., Atacho, D., Steingrímsson, E., & Petersen, P. (2019). Distribution of mast cells within the mouse heart and its dependency on Mitf. Molecular Immunology, 105, 9-15.
dc.identifier.issn 0161-5890
dc.identifier.uri https://hdl.handle.net/20.500.11815/2121
dc.description Publisher's version (útgefin grein)
dc.description.abstract Although mast cell distribution has been described in both human and canine hearts, cardiac mast cells in mice have yet to be categorically localized. We therefore sought to describe mast cell distribution within the mouse heart and characterize their dependence on the Microphthalmia-associated transcription factor (Mitf). Cardiac mast cells were visualized using Toluidine Blue and avidin staining, and their distribution within the heart described. Cardiac mast cells were most prevalent in the epicardium (50%) or myocardium (45%). Less frequently, mast cells were noted in the endocardium (5%). Within the myocardium, 31% of the mast cells had perivascular location. By studying two different Mitf mutant strains, Mitf mi−vga9 and Mitf Mi-wh , we demonstrated that these mutations led to near-complete deficiency of cardiac mast cells. Accordingly, expression of the mMCP-4 and mMCP-5 genes was lost and chymase enzyme activity was severely reduced. Additionally, hearts from mice heterozygous for these Mitf mutations contained significantly fewer mast cells compared to wild-type mice. Our results demonstrated that the distribution of cardiac mast cells in mice is different from humans and dogs. Cardiac mast cells are dependent on Mitf expression, with loss-of-function mutation in the Mitf gene leading to near-complete lack of cardiac mast cells. Loss of a single Mitf allele is sufficient for relative mast cell deficiency.
dc.description.sponsorship This research was supported by grants from the Icelandic Research Fund [grant numbers: 152715-053 and 163068-051]. The funding source had no involvement in the study design, data collection or analysis, the submission process, or any other aspect of the research conduction.
dc.format.extent 9-15
dc.language.iso en
dc.publisher Elsevier BV
dc.relation.ispartofseries Molecular Immunology;105
dc.rights info:eu-repo/semantics/openAccess
dc.subject Chymase
dc.subject Heart
dc.subject Mast cells
dc.subject Mice
dc.subject Microphthalmia-associated transcription factor
dc.subject Hjartað
dc.subject Genarannsóknir
dc.subject MITF
dc.title Distribution of mast cells within the mouse heart and its dependency on Mitf
dc.type info:eu-repo/semantics/article
dcterms.license This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).T
dc.description.version Peer Reviewed
dc.identifier.journal Molecular Immunology
dc.identifier.doi 10.1016/j.molimm.2018.11.009
dc.relation.url https://www.sciencedirect.com/science/article/pii/S0161589018309519?via%3Dihub
dc.contributor.department Læknadeild (HÍ)
dc.contributor.department Faculty of Medicine (UI)
dc.contributor.department Lífvísindasetur (HÍ)
dc.contributor.department Biomedical Center (UI)
dc.contributor.school Heilbrigðisvísindasvið (HÍ)
dc.contributor.school School of Health Sciences (UI)

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