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Stability of thromboxane in blood samples

Stability of thromboxane in blood samples


Title: Stability of thromboxane in blood samples
Author: Helgadottir, Helga   orcid.org/0000-0002-5422-1947
Ólafsson, Ísleifur Hákon
Andersen, Karl Konráð
Gizurarson, Sveinbjorn   orcid.org/0000-0001-7824-9752
Date: 2019-06-04
Language: English
Scope: 143-147
University/Institute: Háskóli Íslands
University of Iceland
School: Heilbrigðisvísindasvið (HÍ)
School of Health Sciences (UI)
Department: Lyfjafræðideild (HÍ)
Faculty of Pharmaceutical Sciences (UI)
Læknadeild (HÍ)
Faculty of Medicine (UI)
Series: Vascular Health and Risk Management;2019(15)
ISSN: 1178-2048
DOI: 10.2147/VHRM.S204925
Subject: Platelet function; Stability; Thromboxane A2; Thromboxane B2; Lyfjaefnafræði; Lyfjagerð
URI: https://hdl.handle.net/20.500.11815/2043

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Citation:

Helgadóttir H, Ólafsson Í, Andersen K, Gizurarson S. Stability of thromboxane in blood samples. Vasc Health Risk Manag. 2019;15:143-147 https://doi.org/10.2147/VHRM.S204925

Abstract:

Introduction: Conventional venous blood collection requires a puncture with a needle through the endothelium of a vessel. The endothelial injury causes activation of circulating platelets and the release of thromboxane A2. The aim of the study was to investigate if platelets continue to form thromboxane A2 in the blood tube after sample collection, but such synthesis would give false information about the actual circulating thromboxane A2 value. Methods: Thromboxane B2 is a biologically inactive but stable metabolite of thromboxane A2 and can be measured in blood samples by a standard enzyme immunoassay. Thromboxane B2 measurements reflect thromboxane A2 concentration. Blood samples were collected in 3.2% sodium citrate vials and EDTA vials from ten individuals and centrifuged and frozen at different time points (0, 30, and 120 minutes). Plasma aliquots were transferred to and frozen in 1.8 mL polypropylene tubes and the citrate samples were also transferred to and frozen in propylene tubes containing indomethacin. Results: Concentrations of thromboxane B2 in plasma samples collected in citrate vials and stored in propylene tubes increased very rapidly as the samples were left for longer after sampling and allowed to stand at room temperature. After 120 minutes, the amount of thromboxane B2 was 400% higher than in the reference sample at time zero. In comparison, thromboxane B2 concentration was about 200% higher in the 120-minute samples compared to the reference in samples collected in citrate vials but stored in indomethacin tubes. In samples collected in EDTA vials, a 10% reduction in thromboxane B2 concentration in the 120-minute samples was observed. Conclusion: Storage conditions, type of sampling vial and time from sampling until sample processing (centrifuging) has a major impact on thromboxane B2 stability.

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