dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Drobnjak, Tijana |
dc.contributor.author |
Jónsdóttir, Anna Margrét |
dc.contributor.author |
Helgadottir, Helga |
dc.contributor.author |
Runólfsdóttir, Margrét Soffía |
dc.contributor.author |
Meiri, Hamutal |
dc.contributor.author |
Sammar, Marei |
dc.contributor.author |
Osol, George J. |
dc.contributor.author |
Mandalà, Maurizio |
dc.contributor.author |
Huppertz, Berthold |
dc.contributor.author |
Gizurarson, Sveinbjorn |
dc.date.accessioned |
2020-09-07T09:09:32Z |
dc.date.available |
2020-09-07T09:09:32Z |
dc.date.issued |
2019-03-27 |
dc.identifier.citation |
Drobnjak T, Jónsdóttir AM, Helgadóttir H, Runólfsdóttir MS, Meiri H, Sammar M, Osol G, Mandalà M, Huppertz B, Gizurarson S. Placental protein 13 (PP13) stimulates rat uterine vessels after slow subcutaneous administration. Int J Womens Health. 2019;11:213-222 https://doi.org/10.2147/IJWH.S188303 |
dc.identifier.issn |
1179-1411 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/2042 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
Introduction: Reduced concentrations of placental protein 13 (PP13) during the first trimester of human pregnancy are associated with elevated risk for the subsequent development of preeclampsia, which is one of the deadliest obstetrical complications of pregnancy. Previous studies by our group have shown that PP13 lowers blood pressure in pregnant rats, increases the size and weight of pups and placentas, and induces vasodilation of resistance arteries through endothelial signaling pathways involving endothelial nitric oxid synthase and prostaglandin. Methods: In the present study, the effect of PP13 was investigated in nonpregnant female Sprague Dawley rats (n=27). Osmotic pumps were surgically implanted subcutaneously that released a constant dose of PP13 or saline over 7 days. Most animals were sacrificed 6 days after the end of PP13 release (on day 13), while some were sacrificed immediately at the end of day 7 (the last PP13 release day), to compare the short-and long-term impact of PP13 on vessels’ growth and size. Results: The uterine vessels were significantly expanded in the group exposed to recombinant PP13 (rPP13) compared to the control (saline) group. Both veins and arteries were significantly expanded by rPP13 with a more pronounced effect after 13 days compared to the corresponding vessels after 7 days. Furthermore, the long-term effect of treatment by rPP13 was more pronounced in the veins compared to the corresponding arteries. The effect of a PP13 variant with a histidine-tag (His-PP13) remained the same between 7 and 13 days. Conclusion: In conclusion, PP13 might play a key role in the expansive remodeling of the uterine vessels, reflecting what would happen if the rat was pregnant, preparing the uterine vasculature for the increase in uteroplacental blood flow, which is necessary for normal pregnancy. We suggest that PP13 could act by NO signaling pathways, a hypothesis that requires future study. |
dc.description.sponsorship |
The authors would like to thank Hy-Laboratories Ltd. for providing PP13 for this study through support provided by the European Union through ASPRE project (#601852). This study was mainly sponsored by Hananja ehf and Icelandic Research Fund (Rannís), grant no 163403-052. The funders had no influence on study design or the analysis of the data. |
dc.format.extent |
213-222 |
dc.language.iso |
en |
dc.publisher |
Informa UK Limited |
dc.relation.ispartofseries |
International Journal of Women's Health;2019(11) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Biomarkers |
dc.subject |
ENOS |
dc.subject |
Galectins |
dc.subject |
Hypertension |
dc.subject |
LGALS13 |
dc.subject |
Placenta |
dc.subject |
Preeclampsia |
dc.subject |
Pregnancy |
dc.subject |
Rat vascular system |
dc.subject |
Slow release |
dc.subject |
Meðganga |
dc.subject |
Fylgja |
dc.subject |
Háþrýstingur |
dc.subject |
Krampi |
dc.subject |
Lyfjagerð |
dc.subject |
Lyfhrifafræði |
dc.title |
Placental protein 13 (PP13) stimulates rat uterine vessels after slow subcutaneous administration |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php
and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you
hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission
for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
International Journal of Women's Health |
dc.identifier.doi |
10.2147/IJWH.S188303 |
dc.relation.url |
https://www.dovepress.com/placental-protein-13-pp13-stimulates-rat-uterine-vessels-after-slow-su-peer-reviewed-article-IJWH |
dc.contributor.department |
Lyfjafræðideild (HÍ) |
dc.contributor.department |
Faculty of Pharmaceutical Sciences (UI) |
dc.contributor.school |
Heilbrigðisvísindasvið (HÍ) |
dc.contributor.school |
School of Health Sciences (UI) |