Noncovalent and site-directed spin labeling of duplex RNA
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Höfundar
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Útgefandi
Royal Society of Chemistry (RSC)
Úrdráttur
An isoindoline-nitroxide derivative of guanine (G, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a G-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, G is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
Lýsing
Post-print (lokagerð höfundar)
Efnisorð
Nicotinamide adenine dinucleotide, Electron paramagnetic resonance, Nucleic acid structure, EPR spectroscopy, Isoindoline nitroxides, Alcohol dehydrogenase, Distance measurements, Abasic sites, DNA, Dynamics, Litrófsgreining, Kjarnsýrur, Rafeindir