Opin vísindi

Pulsed EPR dipolar spectroscopy at Q- and G-band on a trityl biradical

Show simple item record

dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Akhmetzyanov, D.
dc.contributor.author Schöps, P.
dc.contributor.author Marko, A.
dc.contributor.author Kunjir, N. C.
dc.contributor.author Sigurdsson, Snorri
dc.contributor.author Prisner, Thomas F.
dc.date.accessioned 2020-08-10T15:18:45Z
dc.date.available 2020-08-10T15:18:45Z
dc.date.issued 2015
dc.identifier.citation Akhmetzyanov, D., Schöps, P., Marko, A., Kunjir, N. C., Sigurdsson, S. T., & Prisner, T. F. (2015). Pulsed EPR dipolar spectroscopy at Q- and G-band on a trityl biradical. Physical Chemistry Chemical Physics, 17(37), 24446-24451 doi:10.1039/c5cp03671b
dc.identifier.issn 1463-9076
dc.identifier.issn 1463-9084 (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/1958
dc.description Post-print (lokagerð höfundar)
dc.description.abstract Pulsed electron paramagnetic resonance (EPR) spectroscopy is a valuable technique for the precise determination of distances between paramagnetic spin labels that are covalently attached to macromolecules. Nitroxides have commonly been utilised as paramagnetic tags for biomolecules, but trityl radicals have recently been developed as alternative spin labels. Trityls exhibit longer electron spin relaxation times and higher stability than nitroxides under in vivo conditions. So far, trityl radicals have only been used in pulsed EPR dipolar spectroscopy (PDS) at X-band (9.5 GHz), K-u-band (17.2 GHz) and Q-band (34 GHz) frequencies. In this study we investigated a trityl biradical by PDS at Q-band (34 GHz) and G-band (180 GHz) frequencies. Due to the small spectral width of the trityl (30 MHz) at Q-band frequencies, single frequency PDS techniques, like double-quantum coherence (DQC) and single frequency technique for refocusing dipolar couplings (SIFTER), work very efficiently. Hence, Q-band DQC and SIFTER experiments were performed and the results were compared; yielding a signal to noise ratio for SIFTER four times higher than that for DQC. At G-band frequencies the resolved axially symmetric g-tensor anisotropy of the trityl exhibited a spectral width of 130 MHz. Thus, pulsed electron electron double resonance (PELDOR/DEER) obtained at different pump-probe positions across the spectrum was used to reveal distances. Such a multi-frequency approach should also be applicable to determine structural information on biological macromolecules tagged with trityl spin labels.
dc.description.sponsorship The authors acknowledge Dr Vasyl Denysenkov for the technical support with the G-band EPR spectrometer and Dr Alice Bowen for useful discussions and for proof reading the manuscript. This work is supported by SPP 1601 New Frontiers in Sensitivity for EPR Spectroscopy: from Biological Cells to Nano Materials from the German Research Society DFG, the Cluster of Excellence Frankfurt (CEF) Macromolecular Complexes and the Icelandic Research Fund (120001021), which are all gratefully acknowledged.
dc.format.extent 24446-24451
dc.language.iso en
dc.publisher Royal Society of Chemistry (RSC)
dc.relation.ispartofseries Physical Chemistry Chemical Physics;17(37)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Dynamic nuclear polarization
dc.subject Electron double resonance
dc.subject Spin labels
dc.subject Distance measurements
dc.subject Radicals
dc.subject Echo
dc.subject GHZ
dc.subject ESR
dc.subject Algorithm
dc.subject Litrófsgreining
dc.subject Efnagreining
dc.subject Rafeindir
dc.title Pulsed EPR dipolar spectroscopy at Q- and G-band on a trityl biradical
dc.type info:eu-repo/semantics/article
dc.description.version Peer reviewed
dc.identifier.journal Physical Chemistry Chemical Physics
dc.identifier.doi 10.1039/c5cp03671b
dc.relation.url http://pubs.rsc.org/en/content/articlepdf/2015/CP/C5CP03671B
dc.contributor.department Raunvísindadeild (HÍ)
dc.contributor.department Faculty of Physical Sciences (UI)
dc.contributor.department Raunvísindastofnun (HÍ)
dc.contributor.department Science Institute (UI)
dc.contributor.school Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school School of Engineering and Natural Sciences (UI)

Files in this item

This item appears in the following Collection(s)

Show simple item record