dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Gustmann, Henrik |
dc.contributor.author |
Segler, Anna-Lena J |
dc.contributor.author |
Gophane, Dnyaneshwar B |
dc.contributor.author |
Reuss, Andreas J |
dc.contributor.author |
Grünewald, Christian |
dc.contributor.author |
Braun, Markus |
dc.contributor.author |
Weigand, Julia |
dc.contributor.author |
Sigurdsson, Snorri |
dc.contributor.author |
Wachtveitl, Josef |
dc.date.accessioned |
2020-05-28T13:23:01Z |
dc.date.available |
2020-05-28T13:23:01Z |
dc.date.issued |
2018-11-20 |
dc.identifier.citation |
Henrik Gustmann, Anna-Lena J Segler, Dnyaneshwar B Gophane, Andreas J Reuss, Christian Grünewald, Markus Braun, Julia E Weigand, Snorri Th Sigurdsson, Josef Wachtveitl, Structure guided fluorescence labeling reveals a two-step binding mechanism of neomycin to its RNA aptamer, Nucleic Acids Research, Volume 47, Issue 1, 10 January 2019, Pages 15–28 |
dc.identifier.issn |
0305-1048 |
dc.identifier.issn |
1362-4962 (eISSN) |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1856 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
The ability of the cytidine analog Ç m f to act as a position specific reporter of RNA-dynamics was spectroscopically evaluated. Ç m f-labeled single-and double-stranded RNAs differ in their fluorescence lifetimes, quantum yields and anisotropies. These observables were also influenced by the nucleobases flanking Ç m f. This conformation and position specificity allowed to investigate the binding dynamics and mechanism of neomycin to its aptamer N1 by independently incorporating Ç m f at four different positions within the aptamer. Remarkably fast binding kinetics of neomycin binding was observed with stopped-flow measurements, which could be satisfactorily explained with a two-step binding. Conformational selection was identified as the dominant mechanism. |
dc.description.sponsorship |
Deutsche Forschungsgemeinschaft (DFG) through the Collaborative Research Center (CRC) 902; ‘Molecular Principles of RNA-based Regulation’ sub-projects A7, B14 and Mercator Fellowship. Funding for open access charge: DFG (CRC902); sub-projects A7, B14 and Mercator Fellowship. |
dc.format.extent |
15-28 |
dc.language.iso |
en |
dc.publisher |
Oxford University Press (OUP) |
dc.relation.ispartofseries |
Nucleic Acids Research;47(1) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Genetics |
dc.subject |
RNA |
dc.subject |
Erfðafræði |
dc.subject |
DNA-rannsóknir |
dc.title |
Structure guided fluorescence labeling reveals a two-step binding mechanism of neomycin to its RNA aptamer |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
Nucleic Acids Research |
dc.identifier.doi |
10.1093/nar/gky1110 |
dc.relation.url |
http://academic.oup.com/nar/article-pdf/47/1/15/27457606/gky1110.pdf |
dc.contributor.department |
Raunvísindastofnun (HÍ) |
dc.contributor.department |
Science Institute (UI) |
dc.contributor.school |
Verkfræði- og náttúruvísindasvið (HÍ) |
dc.contributor.school |
School of Engineering and Natural Sciences (UI) |