dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Kortesoja, Maarit |
dc.contributor.author |
Karhu, Elina |
dc.contributor.author |
Olafsdottir, Elin Soffia |
dc.contributor.author |
Freysdottir, Jona |
dc.contributor.author |
Hanski, Leena L. |
dc.date.accessioned |
2020-05-19T13:47:34Z |
dc.date.available |
2020-05-19T13:47:34Z |
dc.date.issued |
2019-02-01 |
dc.identifier.citation |
Kortesoja, M. et al., 2019. Impact of dibenzocyclooctadiene lignans from Schisandra chinensis on the redox status and activation of human innate immune system cells. Free Radical Biology and Medicine, 131, pp.309–317. |
dc.identifier.issn |
0891-5849 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1816 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
Redox signaling has been established as an essential component of inflammatory responses, and redox active compounds are of interest as potential immunomodulatory agents. Dibenzocyclooctadiene lignans isolated from Schisandra chinensis, a medicinal plant with widespread use in oriental medicine, have been implicated to possess immunomodulatory properties but their effects on the human innate immune system cells have not been described. In this contribution, data are presented on the impact of schisandrin, schisandrin B and schisandrin C on human monocytic cell redox status, as well as their impact on dendritic cell maturation and T cell activation capacity and cytokine production. In THP-1 cells, levels of intracellular reactive oxygen species (ROS) were elevated after 1 h exposure to schisandrin. Schisandrin B and schisandrin C decreased cellular glutathione pools, which is a phenotype previously reported to promote anti-inflammatory functions. Treatment of human primary monocytes with the lignans during their maturation to dendritic cells did not have any effect on the appearance of surface markers HLA-DR and CD86 but schisandrin B and schisandrin C suppressed the secretion of cytokines interleukin (IL)-6, IL-10 and IL-12 by the mature dendritic cells. Dendritic cells maturated in presence of schisandrin C were further cocultured with naïve CD4+ T cells, resulting in reduced IL-12 production. In THP-1 cells, schisandrin B and schisandrin C reduced the IL-6 and IL-12 production triggered by E. coli lipopolysaccharide and IL-12 production induced by an infection with Chlamydia pneumoniae. In conclusion, the studied lignans act as immunomodulatory agents by altering the cytokine secretion, but do not interfere with dendritic cell maturation. And the observed effects may be associated with the ability of the lignans to alter cellular redox status. |
dc.description.sponsorship |
We would like to acknowledge CIMO (The Centre for International Mobility) for a Nordplus- programme grant for EK. Nina Franko, Milka Lohtaja and Krista Virtanen are acknowledged for excellent technical assistance. We would also like to acknowledge Ilkka Miettinen and Lasse Karhu for excellent assistance in data analysis. |
dc.format.extent |
309-317 |
dc.language.iso |
en |
dc.publisher |
Elsevier BV |
dc.relation.ispartofseries |
Free Radical Biology and Medicine;131 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Dibenzocyclooctadiene lignans |
dc.subject |
Schisandra chinensis |
dc.subject |
Bólgur |
dc.subject |
Lyfjagerð |
dc.subject |
Lyfjaefnafræði |
dc.subject |
Ónæmiskerfi |
dc.title |
Impact of dibenzocyclooctadiene lignans from Schisandra chinensis on the redox status and activation of human innate immune system cells |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
Free Radical Biology and Medicine |
dc.identifier.doi |
10.1016/j.freeradbiomed.2018.12.019 |
dc.relation.url |
https://www.sciencedirect.com/science/article/pii/S0891584918310906?via%3Dihub |
dc.contributor.department |
Lyfjafræðideild (HÍ) |
dc.contributor.department |
Faculty of Pharmaceutical Sciences (UI) |
dc.contributor.department |
Læknadeild (HÍ) |
dc.contributor.department |
Faculty of Medicine (UI) |
dc.contributor.school |
Heilbrigðisvísindasvið (HÍ) |
dc.contributor.school |
School of Health Sciences (UI) |