dc.contributor |
Háskóli Íslands |
dc.contributor |
University of Iceland |
dc.contributor.author |
Mora-Espí, Inmaculada |
dc.contributor.author |
Ibáñez, Elena |
dc.contributor.author |
Soriano, Jorge |
dc.contributor.author |
Nogués, Carme |
dc.contributor.author |
Gudjonsson, Thorarinn |
dc.contributor.author |
Barrios, Leonardo |
dc.date.accessioned |
2020-05-06T13:31:49Z |
dc.date.available |
2020-05-06T13:31:49Z |
dc.date.issued |
2019-04-11 |
dc.identifier.citation |
Mora-Espí, I.; Ibáñez, E.; Soriano, J.; Nogués, C.; Gudjonsson, T.; Barrios, L. Cell Internalization in Fluidic Culture Conditions Is Improved When Microparticles Are Specifically Targeted to the Human Epidermal Growth Factor Receptor 2 (HER2). Pharmaceutics 2019, 11, 177. |
dc.identifier.issn |
1999-4923 |
dc.identifier.uri |
https://hdl.handle.net/20.500.11815/1778 |
dc.description |
Publisher's version (útgefin grein) |
dc.description.abstract |
Purpose: To determine if the specific targeting of microparticles improves their internalization by cells under fluidic conditions. Methods: Two isogenic breast epithelial cell lines, one overexpressing the Human Epidermal Growth Factor Receptor 2 (HER2) oncogene (D492HER2) and highly tumorigenic and the other expressing HER2 at much lower levels and non-tumorigenic (D492), were cultured in the presence of polystyrene microparticles of 1 _m in diameter, biofunctionalized with either a specific anti-HER2 antibody or a non-specific secondary antibody. Mono- and cocultures of both cell lines in static and fluidic conditions were performed, and the cells with internalized microparticles were scored. Results: Globally, the D492 cell line showed a higher endocytic capacity than the D492HER2 cell line. Microparticles that were functionalized with the anti-HER2 antibody were internalized by a higher percentage of cells than microparticles functionalized with the non-specific secondary antibody. Although internalization was reduced in fluidic culture conditions in comparison with static conditions, the increase in the internalization of microparticles biofunctionalized with the anti-HER2 antibody was higher for the cell line overexpressing HER2. Conclusion: The biofunctionalization of microparticles with a specific targeting molecule remarkably increases their internalization by cells in fluidic culture conditions (simulating the blood stream). This result emphasizes the importance of targeting for future in vivo delivery of drugs and bioactive molecules through microparticles. |
dc.description.sponsorship |
This research was funded by the Spanish Ministerio de Ciencia e Innovación (MAT2014-57960-C3-3-R and MAT2017-86357-C3-3-R) and the Generalitat de Catalunya (2017-SGR-503). I.M.E. Thanks to the Spanish Ministerio de Ciencia e Innovación for a predoctoral grant. |
dc.format.extent |
177 |
dc.language.iso |
en |
dc.publisher |
MDPI AG |
dc.relation.ispartofseries |
Pharmaceutics;11(4) |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Biofunctionalization |
dc.subject |
Coculture |
dc.subject |
HER2 |
dc.subject |
Microfluidics |
dc.subject |
Polystyrene μPs |
dc.subject |
Frumurannsóknir |
dc.subject |
lyfja |
dc.title |
Cell Internalization in Fluidic Culture Conditions Is Improved When Microparticles Are Specifically Targeted to the Human Epidermal Growth Factor Receptor 2 (HER2) |
dc.type |
info:eu-repo/semantics/article |
dcterms.license |
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
dc.description.version |
Peer Reviewed |
dc.identifier.journal |
Pharmaceutics |
dc.identifier.doi |
10.3390/pharmaceutics11040177 |
dc.relation.url |
https://www.mdpi.com/1999-4923/11/4/177/pdf |
dc.contributor.department |
Læknadeild (HÍ) |
dc.contributor.department |
Faculty of Medicine (UI) |
dc.contributor.department |
Biomedical Center (UI) |
dc.contributor.department |
Lífvísindasetur (HÍ) |
dc.contributor.school |
Heilbrigðisvísindasvið (HÍ) |
dc.contributor.school |
School of Health Sciences (UI) |