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Genome Sequencing of Pleurozium schreberi: The Assembled and Annotated Draft Genome of a Pleurocarpous Feather Moss

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Pederson, Eric R. A.
dc.contributor.author Warshan, Denis
dc.contributor.author Rasmussen, Ulla
dc.date.accessioned 2020-03-26T11:48:38Z
dc.date.available 2020-03-26T11:48:38Z
dc.date.issued 2019-09-01
dc.identifier.citation Pederson, E., Warshan, D., & Rasmussen, U. (2019). Genome Sequencing of : The Assembled and Annotated Draft Genome of a Pleurocarpous Feather Moss. G3 (Bethesda, Md.), 9(9), 2791-2797.
dc.identifier.issn 2160-1836
dc.identifier.uri https://hdl.handle.net/20.500.11815/1656
dc.description Publisher's version (útgefin grein)
dc.description.abstract The pleurocarpous feather moss Pleurozium schreberi is a ubiquitous moss species which plays a fundamental role in many terrestrial ecosystems, for instance within the boreal forest, the Earth’s largest terrestrial biome, this species plays a significant role in driving ecosystem nitrogen and carbon inputs and fluxes. By hosting dinitrogen (N2)-fixing cyanobacteria, the moss-cyanobacteria symbiosis constitutes the main nitrogen input into the ecosystem and by the high productivity and the low decomposability of the moss litter, P. schreberi contributes significantly to build-up soil organic matter, and therefore long-term C sequestration. Knowledge on P. schreberi genome will facilitate the development of ‘omics’ and system’s biology approaches to gain a more complete understanding of the physiology and ecological adaptation of the moss and the mechanisms underpinning the establishment of the symbiosis. Here we present the de novo assembly and annotation of P. schreberi genome that will help investigating these questions. The sequencing was performed using the HiSeq X platform with Illumina paired-end and mate-pair libraries prepared with CTAB extracted DNA. In total, the assembled genome was approximately 318 Mb, while repetitive elements account for 28.42% of the genome and 15,992 protein-coding genes were predicted from the genome, of which 84.23% have been functionally annotated. We anticipate that the genomic data generated will constitute a significant resource to study ecological and evolutionary genomics of P. schreberi, and will be valuable for evo-devo investigations as well as our understanding of the evolution of land plants by providing the genome of a pleurocarpous moss.
dc.description.sponsorship We would like to thank colleagues at the Oak Ridge National Laboratory, Duke University, and the DOE Joint Genome Institute for access to prepublication genome and annotation of Sphagnum fallax to use in comparisons against gene families. The Nilsson-Ehle Endowments from The Royal Physiographic Society of Lund for financial support. The authors would also like to acknowledge support from Science for Life Laboratory (SciLifeLab, Sweden), the National Genomics Infrastructure (NGI, Stockholm, Sweden), Nation Bioinfor-matics Infrastructure (NBIS; Stockholm, Sweden) and Uppmax (Uppsala, Sweden), for providing assistance in massive parallel sequencing as well as the computational infrastructure.
dc.format.extent 2791-2797
dc.language.iso en
dc.publisher Genetics Society of America
dc.relation.ispartofseries G3: Genes, Genomes, Genetics;9
dc.rights info:eu-repo/semantics/openAccess
dc.subject Annotation
dc.subject Comparative genomic
dc.subject Genome assembly
dc.subject Genome sequencing
dc.subject Pleurozium schreberi
dc.subject Mosadýr
dc.subject Genamengi
dc.subject Genarannsóknir
dc.title Genome Sequencing of Pleurozium schreberi: The Assembled and Annotated Draft Genome of a Pleurocarpous Feather Moss
dc.type info:eu-repo/semantics/article
dcterms.license This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.description.version Peer Reviewed
dc.identifier.journal G3: Genes, Genomes, Genetics
dc.identifier.doi 10.1534/g3.119.400279
dc.relation.url https://syndication.highwire.org/content/doi/10.1534/g3.119.400279
dc.contributor.department Faculty of Life and Environmental Sciences (UI)
dc.contributor.department Líf- og umhverfisvísindadeild (HÍ)
dc.contributor.school Verkfræði- og náttúruvísindasvið (HÍ)
dc.contributor.school School of Engineering and Natural Sciences (UI)

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