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Combined artificial high-silicate medium and LED illumination promote carotenoid accumulation in the marine diatom Phaeodactylum tricornutum

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Yi, Zhiqian
dc.contributor.author Su, Yixi
dc.contributor.author Cherek, Paulina
dc.contributor.author Nelson, David R.
dc.contributor.author Lin, Jianping
dc.contributor.author Rolfsson, Óttar
dc.contributor.author Wu, Hua
dc.contributor.author Salehi-Ashtiani, Kourosh
dc.contributor.author Brynjolfsson, Sigurdur
dc.contributor.author Fu, Weiqi
dc.date.accessioned 2020-02-06T11:05:27Z
dc.date.available 2020-02-06T11:05:27Z
dc.date.issued 2019-12-02
dc.identifier.citation Yi, Z.R. et al., 2019. Combined artificial high-silicate medium and LED illumination promote carotenoid accumulation in the marine diatom Phaeodactylum tricornutum. Microbial Cell Factories, 18(1), p.209.
dc.identifier.issn 1475-2859
dc.identifier.uri https://hdl.handle.net/20.500.11815/1512
dc.description Publisher's version (útgefin grein).
dc.description.abstract Background: Diatoms, which can accumulate large amounts of carotenoids, are a major group of microalgae and the dominant primary producer in marine environments. Phaeodactylum tricornutum, a model diatom species, acquires little silicon for its growth although silicon is known to contribute to gene regulation and play an important role in diatom intracellular metabolism. In this study, we explored the effects of artificial high-silicate medium (i.e. 3.0 mM sodium metasilicate) and LED illumination conditions on the growth rate and pigment accumulation in P.Tricornutum, which is the only known species so far that can grow without silicate. It's well known that light-emitting diodes (LEDs) as novel illuminants are emerging to be superior monochromatic light sources for algal cultivation with defined and efficient red and blue lights. Results: Firstly, we cultivated P.Tricornutum in a synthetic medium supplemented with either 0.3 mM or 3.0 mM silicate. The morphology and size of diatom cells were examined: The proportion of the oval and triradiate cells decreased while the fusiform cells increased with more silicate addition in high-silicate medium; the average length of fusiform cells also slightly changed from 14.33 μm in 0.3 mM silicate medium to 12.20 μm in 3.0 mM silicate medium. Then we cultivated P.Tricornutum under various intensities of red light in combination with the two different levels of silicate in the medium. Higher biomass productivity also achieved in 3.0 mM silicate medium than in 0.3 mM silicate medium under red LED light irradiation at 128 μmol/m2/s or higher light intensity. Increasing silicate reversed the down-regulation of fucoxanthin and chlorophyll a under high red-light illumination (i.e. 255 μmol/m2/s). When doubling the light intensity, fucoxanthin content decreased under red light but increased under combined red and blue (50:50) lights while chlorophyll a content reduced under both conditions. Fucoxanthin accumulation and biomass productivity increased with enhanced red and blue (50:50) lights. Conclusion: High-silicate medium and blue light increased biomass and fucoxanthin production in P.Tricornutum under high light conditions and this strategy may be beneficial for large-scale production of fucoxanthin in diatoms.
dc.description.sponsorship This research was supported by the Icelandic Technology Development Fund (163922-0611), Landsvirkjun Energy Research Fund and NYU Abu Dhabi faculty research funds (AD060).
dc.format.extent 209
dc.language.iso en
dc.publisher Springer Science and Business Media LLC
dc.relation.ispartofseries Microbial Cell Factories;18(1)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Carotenoid
dc.subject Diatoms
dc.subject Fucoxanthin
dc.subject LED light
dc.subject Morphology
dc.subject Silicate
dc.subject Kísilþörungar
dc.subject Ljóstækni
dc.title Combined artificial high-silicate medium and LED illumination promote carotenoid accumulation in the marine diatom Phaeodactylum tricornutum
dc.type info:eu-repo/semantics/article
dcterms.license This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.description.version Peer Reviewed
dc.identifier.journal Microbial Cell Factories
dc.identifier.doi 10.1186/s12934-019-1263-1
dc.contributor.department Læknadeild (HÍ)
dc.contributor.department Faculty of Medicine (UI)
dc.contributor.department Faculty of Industrial Eng., Mechanical Eng. and Computer Science (UI)
dc.contributor.department Iðnaðarverkfræði-, vélaverkfræði- og tölvunarfræðideild (HÍ)
dc.contributor.department Rannsóknarsetur í kerfislíffræði (HÍ)
dc.contributor.department Center for Systems Biology (UI)
dc.contributor.school Heilbrigðisvísindasvið (HÍ)
dc.contributor.school School of Health Sciences (UI)
dc.contributor.school School of Engineering and Natural Sciences (UI)
dc.contributor.school Verkfræði- og náttúruvísindasvið (HÍ)

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