A short isoform of ATG7 fails to lipidate LC3/GABARAP

Ogmundsdottir, Margret H; Fock, Valerie; Sooman, L.; Pogenberg, V.; Dilshat, R.; Bindesbøll, C.; Ögmundsdóttir, Helga M.; Simonsen, A.; Wilmanns, M.; Steingrimsson, Eirikur

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dc.contributor	Háskóli Íslands
dc.contributor	University of Iceland
dc.contributor.author	Ogmundsdottir, Margret H
dc.contributor.author	orcid.org/0000-0002-7812-4340 Fock, Valerie
dc.contributor.author	Sooman, L.
dc.contributor.author	Pogenberg, V.
dc.contributor.author	Dilshat, R.
dc.contributor.author	Bindesbøll, C.
dc.contributor.author	Ögmundsdóttir, Helga M.
dc.contributor.author	Simonsen, A.
dc.contributor.author	Wilmanns, M.
dc.contributor.author	orcid.org/0000-0001-5826-7486 Steingrimsson, Eirikur
dc.date.accessioned	2019-09-30T15:33:22Z
dc.date.available	2019-09-30T15:33:22Z
dc.date.issued	2018-09-26
dc.identifier.citation	Ogmundsdottir, M. H., Fock, V., Sooman, L., Pogenberg, V., Dilshat, R., Bindesbøll, C., . . . Steingrimsson, E. (2018). A short isoform of ATG7 fails to lipidate LC3/GABARAP. Scientific Reports, 8(1), 14391. doi:10.1038/s41598-018-32694-7
dc.identifier.issn	2045-2322
dc.identifier.uri	https://hdl.handle.net/20.500.11815/1272
dc.description	Publisher's version (útgefin grein)
dc.description.abstract	Autophagy is a degradation pathway important for cellular homeostasis. The E1-like enzyme ATG7 is a key component of the autophagy machinery, with the main function of mediating the lipidation of LC3/GABARAP during autophagosome formation. By analysing mRNA-sequencing data we found that in addition to the full-length ATG7 isoform, various tissues express a shorter isoform lacking an exon of 27 amino acids in the C-terminal part of the protein, termed ATG7(2). We further show that ATG7(2) does not bind LC3B and fails to mediate the lipidation of members of the LC3/GABARAP family. We have thus identified an isoform of ATG7 that is unable to carry out the best characterized function of the protein during the autophagic response. This short isoform will have to be taken into consideration when further studying the role of ATG7.
dc.description.sponsorship	This work was supported by a START Marie Curie/Icelandic Research Fund grant (M.H.O.; grant number 120457-041), Icelandic Research Fund grant (M.H.O.; grant number 184727-051), an Icelandic Cancer Society Research Fund grant (M.H.O.), Icelandic Research Fund grant (E.S.; grant number 152715) and by an Erwin Schrödinger fellowship grant from the Austrian Science Fund (V.F.; grant number: J 3864-B26).
dc.format.extent	14391
dc.language.iso	en
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartofseries	Scientific Reports;8(1)
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Macroautophagy
dc.subject	Nutrient signalling
dc.subject	Líffærafræði
dc.subject	Sjálfsát
dc.title	A short isoform of ATG7 fails to lipidate LC3/GABARAP
dc.type	info:eu-repo/semantics/article
dcterms.license	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.description.version	Peer Reviewed
dc.identifier.journal	Scientific Reports
dc.identifier.doi	10.1038/s41598-018-32694-7
dc.contributor.department	Læknadeild (HÍ)
dc.contributor.department	Faculty of Medicine (UI)
dc.contributor.department	Lífvísindasetur (HÍ)
dc.contributor.department	Biomedical Center (UI)
dc.contributor.school	Heilbrigðisvísindasvið (HÍ)
dc.contributor.school	School of Health Sciences (UI)