Opin vísindi

Infraspecific Variation of Huperzine A and B in Icelandic Huperzia selago Complex

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dc.contributor Háskóli Íslands
dc.contributor University of Iceland
dc.contributor.author Xu, Maonian
dc.contributor.author Heiðmarsson, Starri
dc.contributor.author Thorsteinsdóttir, Margrét
dc.contributor.author Wasowicz, Pawel
dc.contributor.author Sun, Hang
dc.contributor.author Deng, Tao
dc.contributor.author Omarsdottir, Sesselja
dc.contributor.author Olafsdottir, Elin Soffia
dc.date.accessioned 2019-02-12T15:32:44Z
dc.date.available 2019-02-12T15:32:44Z
dc.date.issued 2018-10-05
dc.identifier.citation Xu, M., Heidmarsson, S., Thorsteinsdottir, M., Wasowicz, P., Sun, H., Deng, T., . . . Olafsdottir, E. S. (2019). Infraspecific Variation of Huperzine A and B in Icelandic Huperzia selago Complex. Planta Med, 85(02), 160-168. doi:10.1055/a-0752-0295
dc.identifier.issn 0032-0943
dc.identifier.issn 1439-0221 (eISSN)
dc.identifier.uri https://hdl.handle.net/20.500.11815/1022
dc.description Publisher's version (útgefin grein)
dc.description.abstract The alkaloids huperzine A and huperzine B were originally isolated from the Chinese club moss Huperzia serrata. They are known inhibitors of acetylcholinesterase, and especially huperzine A shows pharmaceutical potential for the treatment of Alzheimerʼs disease. Its supply heavily relies on natural plant sources belonging to the genus Huperzia, which shows considerable interspecific huperzine A variations. Furthermore, taxonomic controversy remains in this genus, particularly in the Huperzia selago group. With focus on Icelandic H. selago taxa, we aimed to explore the relatedness of Huperzia species using multi-locus phylogenetic analysis, and to investigate correlations between huperzine A contents, morphotypes, and genotypes. Phylogenetic analysis was performed with five chloroplastic loci (the intergenic spacer between the photosystem II protein D1 gene and the tRNA-His gene, maturase K, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, tRNA-Leu, and the intergenic spacer region between tRNA-Leu and tRNA-Phe). Huperzine A and huperzine B contents were determined using an HPLC-UV method. The phylogenetic analysis suggests that previously proposed Huperzia appressa and Huperzia arctica should not be considered species, but rather subspecies of H. selago. Three genotypes of Icelandic H. selago were identified and presented in a haplotype networking diagram. A significantly (p < 0.05) higher amount of huperzine A was found in H. selago genotype 3 (264 – 679 µg/g) than genotype 1 (20 – 180 µg/g), where the former shows a typical green and reflexed “selago” morphotype. The huperzine A content in genotype 3 is comparable to Chinese H. serrata and a good alternative huperzine A source. Genotype 2 contains multiple morphotypes with a broad huperzine A content (113 – 599 µg/g). The content of huperzine B in Icelandic taxa (6 – 13 µg/g) is much lower than that in Chinese H. serrata (79 – 207 µg/g).
dc.description.sponsorship The People Programme (Marie Curie Actions) of the European Unionʼs Seventh Framework Programme FP7/2007–2013 (grant number 606895) and the Icelandic Research Fund (grant number 152604051) are acknowledged for financial support. This study was also funded from the Bergthora and Thorsteinn Scheving Thorsteinsson Fund.
dc.format.extent 160-168
dc.language.iso en
dc.publisher Georg Thieme Verlag KG
dc.relation info:eu-repo/grantAgreement/EC/FP7/606895
dc.relation.ispartofseries Planta Medica;85(02)
dc.rights info:eu-repo/semantics/openAccess
dc.subject Lycopodiaceae
dc.subject Huperzia
dc.subject Huperzine A
dc.subject Huperzine B
dc.subject Phylogeny
dc.subject Lyfjafræði
dc.subject Lyfjaefnafræði
dc.subject Lyfjagerð
dc.subject Alzheimer sjúkdómur
dc.title Infraspecific Variation of Huperzine A and B in Icelandic Huperzia selago Complex
dc.type info:eu-repo/semantics/article
dc.description.version Peer Reviewed
dc.identifier.journal Planta Medica
dc.identifier.doi 10.1055/a-0752-0295
dc.relation.url http://www.thieme-connect.de/products/ejournals/pdf/10.1055/a-0752-0295.pdf
dc.contributor.department Lyfjafræðideild (HÍ)
dc.contributor.department Faculty of Pharmaceutical Sciences (UI)
dc.contributor.school Heilbrigðisvísindasvið (HÍ)
dc.contributor.school School of Health Sciences (UI)

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