Infraspecific Variation of Huperzine A and B in Icelandic Huperzia selago Complex

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dc.contributorHáskóli Íslandsen_US
dc.contributorUniversity of Icelanden_US
dc.contributor.authorXu, Maonian
dc.contributor.authorHeiðmarsson, Starri
dc.contributor.authorThorsteinsdóttir, Margrét
dc.contributor.authorWasowicz, Pawel
dc.contributor.authorSun, Hang
dc.contributor.authorDeng, Tao
dc.contributor.authorOmarsdottir, Sesselja
dc.contributor.authorOlafsdottir, Elin Soffia
dc.contributor.departmentLyfjafræðideild (HÍ)en_US
dc.contributor.departmentFaculty of Pharmaceutical Sciences (UI)en_US
dc.contributor.schoolHeilbrigðisvísindasvið (HÍ)en_US
dc.contributor.schoolSchool of Health Sciences (UI)en_US
dc.date.accessioned2019-02-12T15:32:44Z
dc.date.available2019-02-12T15:32:44Z
dc.date.issued2018-10-05
dc.descriptionPublisher's version (útgefin grein)en_US
dc.description.abstractThe alkaloids huperzine A and huperzine B were originally isolated from the Chinese club moss Huperzia serrata. They are known inhibitors of acetylcholinesterase, and especially huperzine A shows pharmaceutical potential for the treatment of Alzheimerʼs disease. Its supply heavily relies on natural plant sources belonging to the genus Huperzia, which shows considerable interspecific huperzine A variations. Furthermore, taxonomic controversy remains in this genus, particularly in the Huperzia selago group. With focus on Icelandic H. selago taxa, we aimed to explore the relatedness of Huperzia species using multi-locus phylogenetic analysis, and to investigate correlations between huperzine A contents, morphotypes, and genotypes. Phylogenetic analysis was performed with five chloroplastic loci (the intergenic spacer between the photosystem II protein D1 gene and the tRNA-His gene, maturase K, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, tRNA-Leu, and the intergenic spacer region between tRNA-Leu and tRNA-Phe). Huperzine A and huperzine B contents were determined using an HPLC-UV method. The phylogenetic analysis suggests that previously proposed Huperzia appressa and Huperzia arctica should not be considered species, but rather subspecies of H. selago. Three genotypes of Icelandic H. selago were identified and presented in a haplotype networking diagram. A significantly (p < 0.05) higher amount of huperzine A was found in H. selago genotype 3 (264 – 679 µg/g) than genotype 1 (20 – 180 µg/g), where the former shows a typical green and reflexed “selago” morphotype. The huperzine A content in genotype 3 is comparable to Chinese H. serrata and a good alternative huperzine A source. Genotype 2 contains multiple morphotypes with a broad huperzine A content (113 – 599 µg/g). The content of huperzine B in Icelandic taxa (6 – 13 µg/g) is much lower than that in Chinese H. serrata (79 – 207 µg/g).en_US
dc.description.sponsorshipThe People Programme (Marie Curie Actions) of the European Unionʼs Seventh Framework Programme FP7/2007–2013 (grant number 606895) and the Icelandic Research Fund (grant number 152604051) are acknowledged for financial support. This study was also funded from the Bergthora and Thorsteinn Scheving Thorsteinsson Fund.en_US
dc.description.versionPeer Revieweden_US
dc.format.extent160-168en_US
dc.identifier.citationXu, M., Heidmarsson, S., Thorsteinsdottir, M., Wasowicz, P., Sun, H., Deng, T., . . . Olafsdottir, E. S. (2019). Infraspecific Variation of Huperzine A and B in Icelandic Huperzia selago Complex. Planta Med, 85(02), 160-168. doi:10.1055/a-0752-0295en_US
dc.identifier.doi10.1055/a-0752-0295
dc.identifier.issn0032-0943
dc.identifier.issn1439-0221 (eISSN)
dc.identifier.journalPlanta Medicaen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11815/1022
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag KGen_US
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/606895en_US
dc.relation.ispartofseriesPlanta Medica;85(02)
dc.relation.urlhttp://www.thieme-connect.de/products/ejournals/pdf/10.1055/a-0752-0295.pdfen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLycopodiaceaeen_US
dc.subjectHuperziaen_US
dc.subjectHuperzine Aen_US
dc.subjectHuperzine Ben_US
dc.subjectPhylogenyen_US
dc.subjectLyfjafræðien_US
dc.subjectLyfjaefnafræðien_US
dc.subjectLyfjagerðen_US
dc.subjectAlzheimer sjúkdómuren_US
dc.titleInfraspecific Variation of Huperzine A and B in Icelandic Huperzia selago Complexen_US
dc.typeinfo:eu-repo/semantics/articleen_US

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