Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles

Almenn færsla
dc.contributorHáskóli Íslandsen_US
dc.contributorUniversity of Icelanden_US
dc.contributor.authorAllahgholi, Leila
dc.contributor.authorSardari, Roya R. R.
dc.contributor.authorHakvåg, Sigrid
dc.contributor.authorAra, Kazi Z. G.
dc.contributor.authorKristjánsdóttir, Þórdís
dc.contributor.authorAasen, Inga Marie
dc.contributor.authorFriðjónsson, Ólafur H.
dc.contributor.authorBrautaset, Trygve
dc.contributor.authorHreggvidsson, Gudmundur Oli
dc.contributor.authorKarlsson, Eva Nordberg
dc.date.accessioned2021-01-11T14:16:07Z
dc.date.available2021-01-11T14:16:07Z
dc.date.issued2020-03-30
dc.descriptionPublisher's version (útgefin grein)en_US
dc.description.abstractBrown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining and several processing problems need to be solved to make them a viable option. Pre-treatment is necessary to enhance accessibility and solubility of the biomass components but should be minimal and mild to assure sustainable and cost-effective processing. Here, two routes to pre-treatLaminaria digitata to release polysaccharides were investigated: hot water pre-treatment by autoclaving (121 °C, 20 min or 60 min) and a two-step extraction with mild acid (0.1 M HCl) followed by alkaline treatment. Hot water pre-treatment resulted in partial extraction of a mixture of polysaccharides consisting of alginate, fucoidan and laminarin. After mild acid pre-treatment, alginate was found in the remaining insoluble residues and was extracted in a second step via alkaline treatment using Na2CO3 (0.15 M) at 80 °C and CaCl2 (10%) for the precipitation. In addition to carbohydrates, a fraction of other components such as proteins, phenolic compounds, minerals and trace elements was detected in the extracts. Cultivation of the thermophilic bacterial strains Rhodothermus marinus DSM 16675 and Bacillus methanolicus MGA3 (ATCC 53907) in media supplemented with the respective extracts resulted in growth of both strains, indicating that they were able to utilize the available carbon source for growth. R. marinus displayed the highest cell density in the medium containing the extract from acid pre-treatment, whereas B. methanolicus growth was highest with the extract from hot water pre-treatment.en_US
dc.description.sponsorshipOpen access funding provided by Lund University. The authors received financial support for the projects Thermofactories, (Era-net MBT1, grant agreement No 604814), MacroVal (the Swedish research council Formas, grant 2015-769), Marine food resources for new markets (Formas, grant 2018-01863), Macro cascade (Bio-Based Industries Joint Undertaking under EU Horizon 2020, grant agreement No 720755), the Novo Nordisk foundation (grant agreement No NNF18OC00349792) and ProSeaFood, (Era-net SusFood2, grant agreement No 727473).en_US
dc.description.versionPeer Revieweden_US
dc.format.extent1933-1947en_US
dc.identifier.citationAllahgholi, L., Sardari, R.R.R., Hakvåg, S. et al. Composition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophiles. Journal of Applied Phycology 32, 1933–1947 (2020). https://doi.org/10.1007/s10811-020-02103-6en_US
dc.identifier.doi10.1007/s10811-020-02103-6
dc.identifier.issn0921-8971
dc.identifier.issn1573-5176 (eISSN)
dc.identifier.journalJournal of Applied Phycologyen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11815/2344
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/720755en_US
dc.relation.ispartofseriesJournal of Applied Phycology;32(3)
dc.relation.urlhttps://link.springer.com/article/10.1007%2Fs10811-020-02103-6en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacillus methanolicusen_US
dc.subjectCarbohydrate extractionen_US
dc.subjectLaminaria digitataen_US
dc.subjectMild physicochemical pre-treatmenten_US
dc.subjectRhodothermus marinusen_US
dc.subjectHrossaþarien_US
dc.subjectBrúnþörungaren_US
dc.titleComposition analysis and minimal treatments to solubilize polysaccharides from the brown seaweed Laminaria digitata for microbial growth of thermophilesen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dcterms.licenseOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.en_US

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