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Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity

Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity


Titill: Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity
Höfundur: Schwendner, Petra
Bohmeier, Maria
Rettberg, Petra
Beblo-Vranesevic, Kristina
Gaboyer, Frédéric
Moissl-Eichinger, Christine
Perras, Alexandra K.
Vannier, Pauline
Marteinsson, Viggó   orcid.org/0000-0001-8340-821X
Garcia-Descalzo, Laura
... 10 fleiri höfundar Sýna alla höfunda
Útgáfa: 2018-02-27
Tungumál: Enska
Umfang: 335
Háskóli/Stofnun: Háskóli Íslands
University of Iceland
Svið: Heilbrigðisvísindasvið (HÍ)
School of Health Sciences (UI)
Deild: Matvæla- og næringarfræðideild (HÍ)
Faculty of Food Science and Nutrition (UI)
Birtist í: Frontiers in Microbiology;9
ISSN: 1664-302X
DOI: 10.3389/fmicb.2018.00335
Efnisorð: Sodium chloride; Magnesium sulfate; Metabolome; Compatible solutes; Stress response; Sodium; Natrín; Magnesíum (næringarefni); Efnaskipti; Örverur
URI: https://hdl.handle.net/20.500.11815/746

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Tilvitnun:

Schwendner, P., Bohmeier, M., Rettberg, P., Beblo-Vranesevic, K., Gaboyer, F., Moissl-Eichinger, C., . . . Cockell, C. (2018). Beyond Chloride Brines: Variable Metabolomic Responses in the Anaerobic Organism Yersinia intermedia MASE-LG-1 to NaCl and MgSO4 at Identical Water Activity. Frontiers in Microbiology, 9(335). doi:10.3389/fmicb.2018.00335

Útdráttur:

Growth in sodium chloride (NaCl) is known to induce stress in non-halophilic microorganisms leading to effects on the microbial metabolism and cell structure. Microorganisms have evolved a number of adaptations, both structural and metabolic, to counteract osmotic stress. These strategies are well-understood for organisms in NaCl-rich brines such as the accumulation of certain organic solutes (known as either compatible solutes or osmolytes). Less well studied are responses to ionic environments such as sulfate-rich brines which are prevalent on Earth but can also be found on Mars. In this paper, we investigated the global metabolic response of the anaerobic bacterium Yersinia intermedia MASE-LG-1 to osmotic salt stress induced by either magnesium sulfate (MgSO4) or NaCl at the same water activity (0.975). Using a non-targeted mass spectrometry approach, the intensity of hundreds of metabolites was measured. The compatible solutes L-asparagine and sucrose were found to be increased in both MgSO4 and NaCl compared to the control sample, suggesting a similar osmotic response to different ionic environments. We were able to demonstrate that Yersinia intermedia MASE-LG-1 accumulated a range of other compatible solutes. However, we also found the global metabolic responses, especially with regard to amino acid metabolism and carbohydrate metabolism, to be salt-specific, thus, suggesting ion-specific regulation of specific metabolic pathways.

Leyfi:

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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