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Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus

Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus


Title: Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus
Author: Guðmundsdóttir, Sigríður
Kristmundsson, Árni
Árnason, Ívar Örn
Date: 2017-03-30
Language: English
Scope: 21-30
University/Institute: Háskóli Íslands
University of Iceland
Department: Tilraunastöð í meinafræði að Keldum (HÍ)
Institute for Experimental Pathology, Keldur (UI)
Series: Diseases of Aquatic Organisms;124(1)
ISSN: 0177-5103
1616-1580 (eISSN)
DOI: 10.3354/dao03107
Subject: Renibacterium salmoninarum; Salvelinus alpinus; Challenge methods; Infection state; Diagnostic assays; Arctic charr; Bleikja; Gerlar; Bakteríusjúkdómar
URI: https://hdl.handle.net/20.500.11815/619

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Citation:

Gudmundsdóttir, S., Kristmundsson, Á., & Árnason, Í. (2017). Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus. Diseases of Aquatic Organisms, 124(1), 21-30. doi:10.3354/dao03107

Abstract:

Arctic charr Salvelinus alpinus L. is an important species in Icelandic aquaculture and the most common wild salmonid in Iceland. A study on the course of infection with the bacterium Renibacterium salmoninarum was conducted using 3 different challenge methods in brackish and fresh water. Bacterial isolation, ELISA and PCR tests were used for detection of the bacterium in multiple organ samples. In an experiment, run for 34 wk in brackish water, infection was established by intraperitoneal injection with 5 × 106 colony forming units (CFU) fish-1. There were external and internal symptoms of bacterial kidney disease (BKD) and mortalities between 6 and 13 wk after injection. A cohabitation trial was run simultaneously and infection was well established after 4 wk, as demonstrated by the detection methods applied. Symptoms of BKD were not seen and all but 1 cohabitant survived. In a separate experiment, infection was established by pumping a fixed amount of water from a tank with fingerlings infected by intraperitoneal injection into tanks with naïve fish, in fresh or brackish water, for 6 wk. Fish in the inflow tanks were reared for an additional 3 wk. There were neither macroscopic symptoms nor mortalities. ELISA and PCR tests showed that infection started to take hold after 3 wk. The challenge trials demonstrated that Arctic charr is susceptible to R. salmoninarum. Cohabitation and inflow of water from tanks with infected fish provide useful models for further studies on R. salmoninarum infection acquired in a natural way in Arctic charr.

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Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are un - restricted. Authors and original publication must be credited.

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