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Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs

Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs


Titill: Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs
Höfundur: Guðbrandsson, Jóhannes   orcid.org/0000-0002-2231-0910
Franzdottir, Sigridur Rut   orcid.org/0000-0001-9300-7022
Kristjansson, Bjarni K.   orcid.org/0000-0001-6984-5771
Pashay Ahi, Ehsan   orcid.org/0000-0002-6528-1187
Maier, Valerie H.
Kapralova, Kalina H.
Snorrason, Sigurdur S
Jónsson, Zophonías Oddur   orcid.org/0000-0001-5798-9647
Palsson, Arnar   orcid.org/0000-0002-6525-8112
Útgáfa: 2018-02-07
Tungumál: Enska
Umfang: e4345
Háskóli/Stofnun: Háskóli Íslands
University of Iceland
Háskólinn á Hólum
Hólar University College
Svið: Verkfræði- og náttúruvísindasvið (HÍ)
School of Engineering and Natural Sciences (UI)
Deild: Líf- og umhverfisvísindadeild (HÍ)
Faculty of Life and Environmental Sciences (UI)
Líf- og umhverfisvísindastofnun (HÍ)
Institute of Life and Environmental Sciences (UI)
Lífvísindasetur (HÍ)
Biomedical Center (UI)
Fiskeldis- og fiskalíffræðideild (HH)
Department of Aquaculture and Fish Biology (HUC)
Birtist í: PeerJ;6
ISSN: 2167-8359
DOI: 10.7717/peerj.4345
Efnisorð: Developmental Biology; Evolutionary Studies; Genomics; Molecular Biology; Freshwater Biology; Arctic charr; Þróun lífsins; Erfðafræði; Sameindalíffræði; Vatnalíffræði; Bleikja
URI: https://hdl.handle.net/20.500.11815/584

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Tilvitnun:

Guðbrandsson J, Franzdóttir SR, Kristjánsson BK, Ahi EP, Maier VH, Kapralova KH, Snorrason SS, Jónsson ZO, Pálsson A. (2018) Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs. PeerJ 6:e4345 https://doi.org/10.7717/peerj.4345

Útdráttur:

Phenotypic differences between closely related taxa or populations can arise through genetic variation or be environmentally induced, leading to altered transcription of genes during development. Comparative developmental studies of closely related species or variable populations within species can help to elucidate the molecular mechanisms related to evolutionary divergence and speciation. Studies of Arctic charr (Salvelinus alpinus) and related salmonids have revealed considerable phenotypic variation among populations and in Arctic charr many cases of extensive variation within lakes (resource polymorphism) have been recorded. One example is the four Arctic charr morphs in the ∼10,000 year old Lake Thingvallavatn, which differ in numerous morphological and life history traits. We set out to investigate the molecular and developmental roots of this polymorphism by studying gene expression in embryos of three of the morphs reared in a common garden set-up. We performed RNA-sequencing, de-novo transcriptome assembly and compared gene expression among morphs during an important timeframe in early development, i.e., preceding the formation of key trophic structures. Expectedly, developmental time was the predominant explanatory variable. As the data were affected by some form of RNA-degradation even though all samples passed quality control testing, an estimate of 3′-bias was the second most common explanatory variable. Importantly, morph, both as an independent variable and as interaction with developmental time, affected the expression of numerous transcripts. Transcripts with morph effect, separated the three morphs at the expression level, with the two benthic morphs being more similar. However, Gene Ontology analyses did not reveal clear functional enrichment of transcripts between groups. Verification via qPCR confirmed differential expression of several genes between the morphs, including regulatory genes such as AT-Rich Interaction Domain 4A (arid4a) and translin (tsn). The data are consistent with a scenario where genetic divergence has contributed to differential expression of multiple genes and systems during early development of these sympatric Arctic charr morphs.

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