Title: | Immunophenotypic assessment of clonal plasma cells and B-cells in bone marrow and blood in the diagnostic classification of early stage monoclonal gammopathies : an iSTOPMM study |
Author: |
... 6 more authors Show all authors |
Date: | 2023-12-11 |
Language: | English |
Scope: | 2138626 |
Department: | Faculty of Medicine Other departments |
Series: | Blood Cancer Journal; 13(1) |
ISSN: | 2044-5385 |
DOI: | 10.1038/s41408-023-00944-1 |
Subject: | Gigtarlæknisfræði; Blóðlæknisfræði; B-Lymphocytes; Bone Marrow; Humans; Monoclonal Gammopathy of Undetermined Significance; Multiple Myeloma/diagnosis; Paraproteinemias/diagnosis; Plasma Cells; Smoldering Multiple Myeloma/complications; Waldenstrom Macroglobulinemia |
URI: | https://hdl.handle.net/20.500.11815/4671 |
Citation:Pérez-Escurza , O , Flores-Montero , J , Óskarsson , J Þ , Sanoja-Flores , L , del Pozo , J , Lecrevisse , Q , Martín , S , Reed , E R , Hákonardóttir , G K , Harding , S , Þorsteinsdóttir , S , Rögnvaldsson , S , Löve , Þ J , Durie , B , Kristinsson , S Y & Orfao , A 2023 , ' Immunophenotypic assessment of clonal plasma cells and B-cells in bone marrow and blood in the diagnostic classification of early stage monoclonal gammopathies : an iSTOPMM study ' , Blood Cancer Journal , vol. 13 , no. 1 , 182 , pp. 182 . https://doi.org/10.1038/s41408-023-00944-1
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Abstract:Monoclonal gammopathy of undetermined significance (MGUS) is the earliest discernible stage of multiple myeloma (MM) and Waldenström’s macroglobulinemia (WM). Early diagnosis of MG may be compromised by the low-level infiltration, undetectable to low-sensitive methodologies. Here, we investigated the prevalence and immunophenotypic profile of clonal (c) plasma cells (PC) and/or cB-lymphocytes in bone marrow (BM) and blood of subjects with a serum M-component from the iSTOPMM program, using high-sensitive next-generation flow cytometry (NGF), and its utility in the diagnostic classification of early-stage MG. We studied 164 paired BM and blood samples from 82 subjects, focusing the analysis on: 55 MGUS, 12 smoldering MM (SMM) and 8 smoldering WM (SWM). cPC were detected in 84% of the BM samples and cB-lymphocytes in 45%, coexisting in 39% of cases. In 29% of patients, the phenotypic features of cPC and/or cB-lymphocytes allowed a more accurate disease classification, including: 19/55 (35%) MGUS, 1/12 (8%) SMM and 2/8 (25%) SWM. Blood samples were informative in 49% of the BM-positive cases. We demonstrated the utility of NGF for a more accurate diagnostic classification of early-stage MG.
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Description:Publisher Copyright: © 2023, The Author(s).
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